微粒体分离提取试剂盒  Microsome -北京博迈斯科技发展有限公司

Cat # +Size：K249-50

Size：50 assays

Applications：

Convenient and fast isolation of microsomal fraction from animal tissues Assessment of CYP-mediated drug metabolism and xenobiotic biotransformation Protein profiling of microsomal membrane proteins by SDS-PAGE and Western blot

Features & Benefits：

• Simple & convenient protocol

• The kit provides unique formulations of ready-to-use buffers and reagents to isolate microsomes without the need for ultracentrifugation or sucrose gradient fractionation

Kit Components：

• Homogenization Buffer

• Storage Buffer

• Protease Inhibitor Cocktail

Storage Conditions：-20°C

Shipping Conditions：Gel Pack

USAGE：For Research Use Only! Not For Use in Humans.

Can you use frozen tissues to isolate microsomes?

We recommend using fresh tissues. However, sections frozen immediately after isolation can work too. The frozen tissues should not have undergone multiple rounds of freeze and thaw.

Can I obtain not only microsome fraction but also cytosol fraction with your Microsome Isolation Kit (Cat.# K249-50)?

You can obtain cytosolic fractions from the S9 fraction. The S9 fraction was further centrifuged at >20,000 × g (preferably at 100, 000g) for 30 min to separate the microsomes and cytosolic fraction (Yoshihara and Ohta, 1998). Collect the supernatant at this step, that should be your cytosolic fraction.

Which method for the quantification of total protein amount in microsomes would you recommend?

The protein in the microsomal isolate can be quantified with a BCA assay kit.

What is the exact volume of sample required for this assay?

There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.

Do you have trial sizes of this kit?

Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.

Can we purchase individual components of this kit?

Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.

Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?

Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.