K4799-100? IL-2 Microplate (Item A) coated with anti-mouse IL-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse IL-2) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent, 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. (5x concentrated) ? Detection Antibody IL-2 (Item F), biotinylated anti-mouse IL-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 120x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants IL-2 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse IL-2 in serum, plasma & cell culture supernatants. Detection Range: 1 pg/ml - 40 pg/ml. 100 assays. BioVision’s mouse IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-2. This assay employs an antibody specific for mouse IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF). The minimum detectable dose of IL-2 is typically less than 1 pg/ml. Detection Range: 1 pg/ml – 40 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.