K4800-100? IL-2 Microplate (Item A) coated with anti-rat IL-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant IL-2) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. (5x concentrated) ? Detection Antibody IL-2 (Item F), biotinylated anti-IL-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (rat IL-2 conc. is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants IL-2 (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat IL-2 in serum, plasma & cell culture supernatants. Detection Range: 0.1 ng/ml - 30 ng/ml. 100 assays. BioVision’s rat IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2. This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The minimum detectable dose of IL-2 is typically less than 0.1 ng/ml. Detection Range: 0.1 ng/ml – 30 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.