K4903-100? Antibody coated 96-well plate (12x8 well strips) ? 10X Wash concentrate, 50 ml ? 5X Diluent, 50 ml ? Secondary antibody, 12 ml ? 100X Detector (HRP conjugated mouse IgG), 150 μl ? Standard (recombinant rat adiponectin from HEK 293cells), 1 vial, lyophilized (48 ng) ? QC sample= a positive control of rat serum adiponectin, 1 vial, lyophilized (12.67 μg) ? Substrate I, 6 ml ? Substrate II, 6 ml ? Stop solution, 12 mlCell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluidsRat Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in rat Serum, Plasma or Various Tissue or Cell Culture supernatants. 100 Assays.Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Rat Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in rat serum, plasma or various tissue or cell culture supernatants. In the assay, polyclonal antibody specific for rat adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and any adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-rat adiponectin monoclonal antibody. With adding HRP conjugated mouse IgG and HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.