K3560-100? Aromatase Ab-coated plate ? Rat Aromatase standard, lyophilized ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from rat tissues or cells ? Serum, plasma (EDTA, Heparin) Aromatase (Cytochrome P450 19A1) rat ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of rat Aromatase in tissues, cells, plasma, serum or microsomes. Sensitivity: 1.56 ng/ml. Detection Range: 1.56 ng/ml – 100 ng/ml. 100 assays. Aromatase (Cytochrome P450 19A1 or CYP19A1) is a monooxygenase anchored to the Endoplasmic reticulum membrane, its expression being highest in steroidogenic tissue (gonads, breast, adipose tissue, brain, placenta etc.). Aromatase is responsible for conversion of androgens into estrogens and is important in normal sexual development as well as breast, endometrial and other cancers. BioVision’s rat Aromatase ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat Aromatase-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to Aromatase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the rat Aromatase captured onto the plate. The concentration of Aromatase in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 1.56 ng/ml – 100 ng/ml. Sensitivity: 1. 56 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%. Recovery: Serum (~90%), plasma (EDTA = ~90%, Heparin = ~85%).