K4757-100? Serum & plasma ? Cell culture media etc. C-Peptide (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of C-Peptide in Serum, Plasma, Cell culture supernatants etc. Detection range: 0.1-1000 ng/ml. 100 assays.BioVision’s C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- C-Peptide antibody, both biotinylated C-Peptide peptide and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide peptide can be established and the concentration of C-Peptide peptide in the samples can be calculated accordingly. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain. The minimum detectable concentration of C-Peptide is 772 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.