K3831-100? 96 wells coated with anti-rat Estradiol antibody, 1 Microplate with 2 adhesive strips ? Rat Estradiol standard (72 ng/L) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Serum & plasma (EDTA/Citrate), urine ? Cell culture medium, tissue homogenates and cell lysates Estradiol E2 (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat Estradiol (E2 or 17β-estradiol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:2 – 50 ng/L. 100 assays. Estradiol (E2 or 17β-estradiol) is the predominant female reproductive hormone secreted by the ovaries. Smaller amounts of estradiol are also produced by the adrenal cortex and by the testes. BioVision’s rat Estradiol E2 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat Estradiol E2 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Estradiol E2 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat Estradiol E2 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 2 – 50 ng/L.