K4780-100? TNF-beta Microplate (Item A) coated with anti-human TNF-beta, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human TNF-beta) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody TNF-beta (Item F), biotinylated anti-human TNF-beta (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 700x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human TNF-beta concentration is pretty low in normal serum & plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine TNF-beta (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TNF-beta in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 60 pg/ml - 18000 pg/ml. 100 assays. BioVision’s Human TNF-beta (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-beta. This assay employs an antibody specific for human TNF-beta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL- 12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TPO, VEGF. The minimum detectable dose of TNF-beta is typically less than 60 pg/ml. Detection Range: 60 pg/ml – 18,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.