K7212-100? VCAM-1 Microplate coated with anti-mouse monoclonal Ab against VCAM-1, 96 wells ? Lyophilized recombinant mouse VCAM-1 standard (10 ng/vial) ? Biotinylated anti- mouse VCAM-1antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatantsVCAM-1 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse VCAM-1 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s mouse vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for mouse VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VCAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 5 pg/ml.