biovision K139-25 Cell and tissue lysates ? Detection method- Fluorescence (Ex/Em 400/505) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-12 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-12 Activity within 1-2 hrs. Kit Size: 25 and 100 assays. Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.