biovision K183-25 Live cells ? Detection method- Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex. = 485 nm and Em. = 535 nm) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25 and 100 assays) ? Applications- Sensitive detection of activated caspase-3 in living cells. CaspGLOWTM Fluorescein Active Caspase-3 Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspases-3 in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader. Kit Size: 25 and 100 Assays. Activation of caspases plays a central role in apoptosis. The CaspGLOW? Fluorescein Active Caspase-3 Staining Kit provides a convenient means for sensitive detection of activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor, DEVD-FMK, conjugated to FITC (FITC-DEVD-FMK) as a marker. FITC-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.