This kit provides everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. The procedure is efficient and streamlined and can process up to 12 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic proteins from 100 mL of induced bacterial culture. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
该试剂盒提供了从诱导细菌培养物中分离和纯化包涵体蛋白所需的一切。首先，使用专有的细胞裂解试剂选择性裂解细胞并释放固体形式的包涵体。接下来，使用提供的IB溶解试剂溶解包涵体并释放其内含物。然后使用旋转柱进一步纯化包涵体蛋白，以进行快速方便的缓冲液交换和脱盐。该程序高效且精简，可在大约2小时内处理多达12个样本。每个旋转柱能够从100毫升诱导的细菌培养物中回收多达12毫克的酸性或碱性蛋白质。纯化的重组蛋白然后准备用于SDS-PAGE、2D凝胶、蛋白质印迹、质谱分析和其他应用。
Cell Lysis Reagent和IB Solubilization Reagent储存在4°C。所有其他未开封的溶液应在室温下储存。一旦打开，溶液应储存在4°C，除了碱性和酸性结合缓冲液，应在室温下储存。具体保存情况收到产品后请按说明书存放。
