K159-100
biovision K159-100 Cell samples treated with Caspase inhibitor drugs ? Detection method- Fluorescence (Ex/Em 400/505) ? Species reactivity- Mammalian ? Kit size- 100 assays ? Applications- Effective means for screening caspase inhibitors using fluorometric methods. Caspase-9 Inhibitor Drug Screening Kit: Rapid & Convenient Fluorometric Assay to Screen Caspase-9 Inhibitors. Includes Inhibitor Control, Z-VAD-FMK. 100 Assays. Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-9 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEHD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-9 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-9 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors. The assay is simple, straightforward, and can be performed directly in microtiter plates. Each kit contains 100 units of active caspase-9, sufficient for screening 100 caspase inhibitor samples. Assay conditions have been optimized to obtain the maximal activity.
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K118-100
biovision K118-100 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-9 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-9 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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K118-200
biovision K118-200 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-9 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-9 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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K118-25
biovision K118-25 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-9 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-9 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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