|
K109-100
biovision K109-100 Living cells (suspension and adherent) ? Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-Biotin Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.
More
K109-25
biovision K109-25 Living cells (suspension and adherent) ? Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-Biotin Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.
More
K109-400
biovision K109-400 Living cells (suspension and adherent) ? Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-Biotin Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.
More
K128-100
biovision K128-100 Live cells ? Detection method- Flow cytometry (Ex = 488 nm; Em = 578 nm) and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-PE Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis within 10 min. No need to fix the cells. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
More
K128-25
biovision K128-25 Live cells ? Detection method- Flow cytometry (Ex = 488 nm; Em = 578 nm) and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-PE Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis within 10 min. No need to fix the cells. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
More
K128-400
biovision K128-400 Live cells ? Detection method- Flow cytometry (Ex = 488 nm; Em = 578 nm) and fluorescence microscopy ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Annexin V-PE Apoptosis Detection Kit: Simple One Step Assay to Detect Apoptosis within 10 min. No need to fix the cells. Detection: Flow Cytometry or Fluorescence Microscopy The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
More
K203-100
biovision K203-100 Live cells ? Detection method- Flow cytometry using FL1 channel for SYTOX Green dye (Ex = 488 nm; Em = 530 nm) and FL2 channel for Annexin V-PE (Ex = 488 nm; Em = 578 nm). ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100 and 400 assays) ? Applications- Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. PE shows brighter red fluorescence. Sytox green dye is also included to control necrosis. Annexin V-PE Apoptosis Detection Kit Plus: Simple One Step Assay to Detect Apoptotic, Necrotic & live cells within 10 min. No need to fix the cells. Detection: Flow Cytometry or fluorescence microscopy. The Annexin V-PE Apoptosis Detection Kit Plus is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation. The Annexin V-PE Apoptosis Detection Kit Plus includes annexin V-PE, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex. 488 nm/Em. 578 nm).
More
|
|