K125-400
biovision K125-400 Cell and tissue lysates ? Detection method- Absorbance (400 or 405 nm) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-10 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-10 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate AEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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K139-100
biovision K139-100 Cell and tissue lysates ? Detection method- Fluorescence (Ex/Em 400/505) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-12 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-12 Activity within 1-2 hrs. Kit Size: 25 and 100 assays. Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
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K139-25
biovision K139-25 Cell and tissue lysates ? Detection method- Fluorescence (Ex/Em 400/505) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-12 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-12 Activity within 1-2 hrs. Kit Size: 25 and 100 assays. Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
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K152-100
biovision K152-100 Cell samples treated with Caspase inhibitor drugs ? Detection method- Fluorescence (Ex/Em 400/505) ? Species reactivity- Mammalian ? Kit size- 100 assays ? Applications- Effective means for screening caspase inhibitors using fluorometric methods. Caspase-2 Inhibitor Drug Screening Kit: Rapid & Convenient Fluorometric Assay to Screen Caspase-2 Inhibitors. Includes Inhibitor Control, Z-VAD-FMK. 100 Assays. Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-2 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VDVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-2 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-2 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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K116-100
biovision K116-100 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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K116-200
biovision K116-200 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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K116-25
biovision K116-25 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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K116-400
biovision K116-400 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
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K117-100
biovision K117-100 Cell and tissue lysates ? Detection method- Absorbance (400 or 405 nm) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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K117-200
biovision K117-200 Cell and tissue lysates ? Detection method- Absorbance (400 or 405 nm) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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K117-25
biovision K117-25 Cell and tissue lysates ? Detection method- Absorbance (400 or 405 nm) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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K117-400
biovision K117-400 Cell and tissue lysates ? Detection method- Absorbance (400 or 405 nm) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-2 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-2 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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K153-100
biovision K153-100 Cell samples treated with Caspase inhibitor drugs ? Detection method- Fluorescence (Ex/Em 400/505) ? Species reactivity- Mammalian ? Kit size- 100 assays ? Applications- Effective means for screening caspase inhibitors using fluorometric methods. Caspase-3 Inhibitor Drug Screening Kit: Rapid & Convenient Fluorometric Assay to Screen Caspase-3 Inhibitors. Includes Caspase-3 Inhibitor. 100 Assays. Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. The Caspase-3 Inhibitor Drug Screening Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-3 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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K105-100
biovision K105-100 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-3/CPP32 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-3/CPP32 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.
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K105-200
biovision K105-200 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-3/CPP32 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-3/CPP32 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.
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