中文名称:甘油醛3-磷酸测定试剂盒(荧光法)
英文名称:Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric)
货号:K2018-100
规格:100次
供应商:北京博迈斯科技发展有限公司
试剂盒简介:
甘油醛3-磷酸(ga3p)又称磷酸三糖或3-磷酸甘油醛,是一种重要的代谢物,是糖酵解和糖异生等多种生化途径的中间产物。它是在糖酵解过程中由果糖二磷酸醛缩酶分解1,6-二磷酸果糖形成的。它也作为色氨酸生物合成途径中的副产物或硫胺素生物合成途径中的反应物出现。在植物中,ga3p是calvin循环的产物,是合成其他碳水化合物的起始分子。BioVision的甘油醛3-磷酸检测试剂盒是一种稳健的、基于一步平板法的生物样品中GA3P的测定方法。Ga3P显影剂通过释放NADH氧化GA3P,其被Ga3P酶混合物用于将非荧光Ga3P探针转化为在EX/EM=535/587 nm处测量的荧光产物。
检测方法 :荧光检测法(ex/em535/587nm)
物种反应性 :全部
应用 :3-磷酸甘油醛的定量
特点和优点:
•强健
•简单
•一步平板法测定生物样品中的GA3P
试剂盒组成成分:
GA3P显影剂
P酶混合物
GA3P标准品
GA3P探针
ga3p分析缓冲液
储存条件 :-20摄氏度
装运条件:蓝冰运输
用法 :仅供科研研究使用。
Cat :K2018-100
Size:100 assays
Kit Summary:
Glyceraldehyde 3-phosphate (GA3P), also known as triose phosphate or 3-phosphoglyceraldehyde is an important metabolite that occurs as an intermediate central to several biochemical pathways such as glycolysis and gluconeogenesis. It is formed during glycolysis by the breakdown of fructose-1,6-biphosphate by the enzyme fructose biphosphate aldolase. It also occurs as a byproduct in tryptophan biosynthesis pathway or as a reactant in thiamine biosynthesis pathway. In plants, GA3P is the product of the Calvin Cycle and is the starting molecule for the synthesis of other carbohydrates. BioVision’s Glyceraldehyde 3-Phosphate assay kit is a robust, one step plate based assay for the measurement of GA3P in biological samples. The GA3P developer oxidizes GA3P with the release of NADH, which is used by the GA3P enzyme mix to convert the non-fluorescent GA3P probe to a fluorescent product measured at Ex/Em = 535/587 nm.
Detection Method:Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity:All
Applications:Quantification of Glyceraldehyde 3-phosphate
Features & Benefits:
• Robust
• Simple
• One step plate based assay for the measurement of GA3P in biological samples
Kit Components:
GA3P Developer
GA3P Enzyme Mix
GA3P Standard
GA3P Probe
GA3P Assay Buffer
Storage Conditions:-20°C
Shipping Conditions:Gel Pack