K4756-100? Serum & plasma ? Cell culture media etc. Glucagon (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of Glucagon in Serum, Plasma, Cell culture supernatants etc.. Detection range: 1-1000 pg/ml. 100 assays.BioVision’s Glucagon Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Glucagon antibody, both biotinylated Glucagon peptide and peptide standard or targeted peptide in samples interacts competitively with the Glucagon antibody. Uncompeted (bound) biotinylated Glucagon peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Glucagon peptide in the standard or samples. This is due to the competitive binding to Glucagon antibody between biotinylated Glucagon peptide and peptides in standard or samples. A standard curve of known concentration of Glucagon peptide can be established and the concentration of Glucagon peptide in the samples can be calculated accordingly. The minimum detectable concentration of Glucagon is 0.97 pg/ml. The detection range for the kit is 1-1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.