K4774-100? IFN-gamma Microplate (Item A) coated with anti-human IFN-gamma, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IFN-gamma) ? Sample Diluent Buffer (Item D) for Standard/Sample (cell lysate/tissue lysate) diluent (5x concentrated) ? Assay Diluent (Item E) for Detection Antibody & HRP-Streptavidin concentrate diluent (5x concentrated) ? Detection Antibody IFN-γ (Item F), biotinylated anti-human IFN-gamma (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Cell Lysate buffer (Item J) (2x concentrated) ? Cell lysates ? Tissue lysates IFN-gamma (human) ELISA Kit (For Lysates): Colorimetric Assay for Quantitative measurement of human IFN-gamma in cell & tissue lysates. Detection limit: 15 pg/ml. 100 assays. BioVision’s Human IFN-gamma ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-gamma. This assay employs an antibody specific for human IFN-gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti- human IFN-gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IFN-gamma is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, MCP1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.