K4761-100? Plate coated with Irisin Recombinant Protein (12 x 8-well strips) ? Wash Buffer 10X (50 ml) ? Diluent 5X (50 ml) ? Detection Antibody (12 ml) ? Detector 100X (HRP Conjugated anti-rabbit IgG) (150 μl) ? Irisin Standard (lyophilized) (5 μg) ? Irisin QC sample (lyophilized) ? TMB Substrate Solution (12 ml) ? Stop Solution (12 ml) ? Plate sealers (plastic film) ? Serum & plasma ? Cell culture supernatants Irisin Competitive ELISA Kit: Colorimetric Assay for Quantitative Determination of Irisin (human, mouse, rat & monkey) in Serum, Plasma & Cell culture supernatants. Detection Limit: 1 ng/ml. 100 assays. BioVision’s Irisin (human) Competitive ELISA kit is to be used for the in vitro quantitative determination of human irisin in cell culture supernatants, serum and plasma. It should also work for the in vitro quantitative determination of irisin in mouse, rat and monkey biological samples. A polyclonal antibody recognizing native irisin reacts with a series of predetermined recombinant irisin standard proteins or samples under competition in the irisin-coated plate. Their relative reactivity is plotted with that of the standard proteins. Detection limit: 1 ng/ml. Note: The Limit of detection was measured by adding two standard deviations to the mean value of 50 zero standard. Assay Range: 0.001 ug/ml – 5 ug/ml. Irisin levels range in human plasma and serum from 0.2 to > 2 ug /ml.