K4743-100? MMP-8 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-rat MMP-8. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant rat MMP-8. ? Assay Diluent A (Item D): 30 ml of diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody MMP-8 (Item F): 2 vial of biotinylated anti-MMP-8 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 160x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma (using heparin as an anticoagulant, EDTA & Citrate are not recommended) ? Cell culture supernatants ? Urine MMP-8 (rat) ELISA Kit: Colorimetric Assay for Quantitative Determination of rat MMP-8 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 50 pg/ml. 100 assays.BioVision’s Rat MMP-8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP-8. This assay employs an antibody specific for MMP-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of MMP-8 is typically less than 50 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α.