K4711-100? NRG-1 Ab coated plate ? Human NRG-1 Standard ? Detection antibody (100x) ? HRP Conjugate (100x) ? Sample diluent buffer ? Antibody diluent buffer ? HRP diluent buffer ? TMB substrate ? Stop solution ? Serum and plasma ? Cell culture supernatant Neuregulin-1/NRG1-beta1 (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of native and recombinant human Neuregulin-1 in serum, plasma & cell culture supernatant. Detection range: 62.5 pg/ml – 4000 pg/ml. 100 assays. BioVision’s human NRG-1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human NRG-1 is coated on a 96-well plate. Standards and test samples are added to the wells and NRG-1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal detection antibody from goat specific for NRG-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, HRP conjugate is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is used by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human NRG-1 captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 10 pg/ml. Coefficient of variation: 5% (Intra-assay) and 7% (Inter-assay).