K4912-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Human RBP4 Standard (lyophilized, 50 ng) ? Human RBP4 QC Sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? Plate Sealers Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsRBP4 (human) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human RBP4 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.39 – 25 ng RBP4/ml and Detection limit 380 pg/ml. 100 Assays. Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (human) ELISA Kit is to be used for the in vitro quantitative determination of human RBP4 in biological fluids. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3’,5,5’-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-33, human GPX3, human progranulin, human FABP4, human ANGPTL3, human ANGPTL4, human ANG1, human ANG2, human Nampt, mouse Nampt. The assay range is 0.39 – 25 ng RBP4/ml and a detection limit of 380 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).