K4339-100? TGF-alpha Microplate coated with anti-human Ab against TGF-alpha, 96 wells ? Lyophilized recombinant human TGF-alpha standard (10 ng/vial) ? Biotinylated anti-human TGF-alpha antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA) ? Cell culture supernatants ? Human milk TGF-alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TGF-alpha in serum, plasma, cell culture supernatants & human milk. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.BioVision’s human TGF-alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for TGF-alpha coated on a 96-well plate. Standards and test samples are added to the wells and TGF-alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TGF-alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-alpha captured onto the plate. This ELISA kit shows cross-reactivity with TGFβ2, TGFβ3 & TGFβ5 <1%. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.