K7421-100? Plate coated with T4 Ab ? T4 Standard ? Assay Diluent ? T4 Enzyme Conjugate Conc. ? Wash Concentrate (20X) ? TMB Substrate ? Stop SolutionSerum or plasma Thyroxine (T4) (Mouse/Rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of mouse/rat thyroxine in serum or plasma. Sensitivity: 0.467 μg/dl. 100 assays. T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s mouse/rat thyroxine (T4) kit is a solid phase competitive ELISA Kit. The samples, and T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.