K4753-100? Serum & plasma ? Cell culture supernatants TPO (Mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse TPO in Serum, Plasma & Cell culture supernatants. Detection Limit: less than 30 pg/ml. 100 assays.BioVision’s Mouse TPO ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TPO. This assay employs an antibody specific for mouse TPO coated on a 96-well plate. Standards and samples are pipetted into the wells and TPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TPO is typically less than 30 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.