K4766-100? BLC Microplate (Item A) coated with anti-human BLC, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human BLC) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody BLC (Item F), biotinylated anti-human BLC (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Human BLC/BCA-1/CXCL13 ELISA Kit: Colorimetric Assay for Quantitative Determination of human BLC/BCA-1/CXCL13 in Serum, Plasma, Urine & Cell culture supernatants. Detection Range: 1.5 pg/ml - 1000 pg/ml. 100 assays. BioVision’s Human BLC/BCA-1/CXCL13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BLC. This assay employs an antibody specific for human BLC coated on a 96-well plate. Standards and samples are pipetted into the wells and BLC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BLC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BLC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of BLC is typically less than 1.5 pg/ml. Detection Range: 1.5 pg/ml - 1000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.