biovision K114-25 Cell and tissue lysates ? Detection method- fluorecence (400 nm excitation filter and 505 nm emission filter) ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25, 100, 200, 400 assays) ? Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Caspase-6 Activity Fluorometric Assay Kit: Simple & Convenient fluorometric Assay to Measure Caspase-6 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays. Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-6 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on detection of cleavage of substrate VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VEID-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by Mch2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.