biovision K190-25 Live cells ? Detection method- Fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm) and Flow cytometry ? Species reactivity- Mammalian ? Kit size- Convenient sizes (25 and 100 assays) ? Applications- Detection of activated caspases in living cells. CaspGLOWTM Red Active Caspase Staining Kit: Convenient & Sensitive Kit to Detect Activated Caspases in Living Cells. Detection Method: Fluorescence Microscopy, Flow Cytometry or Fluorescence Plate Reader. Kit Size: 25 and 100 Assays. Activation of caspases plays a central role in apoptosis. The CaspGLOWTM Red Active Caspase Staining Kit provides a convenient means for detecting activated caspases in living cells. The assay utilizes a caspase family inhibitor VAD-FMK conjugated to sulfo-rhodamine (Red-VAD-FMK) as the fluorescent marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells.