IL-33 Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot, ELISA, Neutralization, Immunohistochemistry 1) WB: Use 0.1-0.2 μg/ml. The detection limit for recombinant human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.
4) Immunohistochemistry: 0.125 μg/ml-0.25 μg/ml with an overnight incubation at 4°C