Overview

The only kit for easy isolation of bacterial outer membrane vesicles (OMVs)

Expanding our exosome expertise to bacteria, SBI now offers the ExoBacteria^TM OMV Isolation Kit. The first and only kit designed to streamline isolation of bacterial outer membrane vesicles (OMVs), the ExoBacteria OMV Isolation Kit uses an innovative, precipitation-free gravity column system to harvest OMVs from bacterial culture medium. With OMVs in less than 1-hour and delivering a purity and yield rivaling ultracentrifugation-based approaches, the ExoBacteria OMV Isolation Kit is a great way to accelerate your studies on bacterial communication and pathogenesis¹, cancer therapy², bacterial modulation of the host immune response³, and bacterial OMV engineering for use as vaccines

• Unique—the only dedicated isolation system for bacterial OMVs
• Fast—go from cultured bacterial media to purified OMV in less than 1 hour, twice as fast as ultracentrifugation
• Simple—easy-to-setup, precipitation-free gravity column system
• Better—get significantly higher yields and purity compared to ultracentrifugation-based approaches

Each kit comes with enough reagents and consumables to process up to 20 reactions*, enabling maximal flexibility with experimental conditions.

*1 reaction is defined as 30 ml of purified bacterial culture media loaded into a single gravity column.

How It Works

Demystifying OMV Isolation: A newer, faster, simpler method with high yields and purity

1. Clarify up to 30 ml of bacteria culture media
2. Pour supernatant into ExoBacteria OMV isolation column
3. Wash column with 15 ml of binding buffer
4. Elute bacterial OMVs with 1.5 ml of elution buffer