线粒体/胞浆分离提取试剂盒 Mitochondria/Cytosol -北京博迈斯科技发展有限公司

Detection Method:Western blotting, ELISA, or other assays.

Species Reactivity:Mammalian

Applications:Effective isolation of a highly enriched mitochondrial fraction from cytosolic fraction of mammalian cells including both apoptotic and nonapoptotic cells.

Features & Benefits:

• Simple procedure; takes only 3-4 hours

• Fast and convenient

• The fractionation procedure is simple and staightforward. No ultracentrifugations are required. No toxic chemicals are involved.

Kit Components:

• Mitochondria Extraction Buffer

• 5X Cytosol Extraction Buffer

• DTT (1 M)

• Protease Inhibitor Cocktail

Storage Conditions:-20°C

Shipping Conditions:Gel Pack

USAGE:For Research Use Only! Not For Use in Humans.

参考文献：

Ian T. Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways. Cancer Cell Int, April 2019; 31011292.

Keunjung Heo. A De Novo RAPGEF2 Variant Identified in a Sporadic Amyotrophic Lateral Sclerosis Patient Impairs Microtubule Stability and Axonal Mitochondria Distribution. Exp Neurobiol, Dec 2018; 30636905.

Wang, X. et al., (2017) Geraniin suppresses ovarian cancer growth through inhibition of NF‐κB activation and downregulation of Mcl‐1 expression, Journal of Biochemical and Molecular Toxicology, Jun.2017, 101002/jbt21929

Marni E. Cueno et al., (2017) Periodontal disease level-butyric acid putatively contributes to the ageing blood: A proposed link between periodontal diseases and the ageing process, Mechanisms of Aging and Development, 2017, 162:100-105

Ping-Yi Lin et al., (2017) Chlorella sorokiniana induces mitochondrial-mediated apoptosis in human non-small cell lung cancer cells and inhibits xenograft tumor growth in vivo, BMC Complementary and Alternative Medicine, 2017,