K4797-100
K4797-100? IL-1 alpha Microplate (Item A) coated with anti-rat IL-1 alpha, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant rat IL-1 alpha) ? Assay Diluent C (Item L) for Standard/Sample (serum/plasma/cell culture medium) diluent ? Assay Diluent B (Item E) for detection antibody & HRP-streptavidin diluent. (5x concentrated) ? Detection Antibody IL-1 alpha (Item F), biotinylated anti-rat IL-1 alpha (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants IL-1α (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat IL-1 alpha in serum, plasma & cell culture supernatants. Detection Range: 15 pg/ml - 8000 pg/ml. 100 assays. BioVision’s rat IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 alpha. This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The minimum detectable dose of IL-1 alpha is typically less than 15 pg/ml. Detection Range: 15 pg/ml – 8000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4794-100
K4794-100? IL-1 beta Microplate (Item A) coated with anti-human IL-1 beta, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IL-1 beta) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody IL-1 beta (Item F), biotinylated anti-human IL-1 beta (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 300x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human IL-1 beta conc. is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine IL-1β (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IL-1 beta in serum, plasma, urine & cell culture supernatants. Detection Range: 0.3 pg/ml - 100 pg/ml. 100 assays. BioVision’s Human IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 beta. This assay employs an antibody specific for human IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of IL-1 beta is typically less than 0.3 pg/ml. Detection Range: 0.3 pg/ml – 100 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4795-100
K4795-100? IL-1 beta Microplate (Item A) coated with anti-mouse IL-1 beta, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse IL-1 beta) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent (5x concentrated) ? Detection Antibody IL-1 beta (Item F), biotinylated anti-mouse IL-1 beta (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants IL-1β (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse IL-1 beta in serum, plasma & cell culture supernatants. Detection Range: 5 pg/ml - 2000 pg/ml. 100 assays. BioVision’s mouse IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 beta. This assay employs an antibody specific for mouse IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1α, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13,IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 5 pg/ml. Detection Limit: 5 pg/ml – 2000 pg/ml.
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K4796-100
K4796-100? IL-1 beta Microplate (Item A) coated with anti-rat IL-1 beta, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant IL-1 beta) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent (5x concentrated) ? Detection Antibody IL-1 beta (Item F), biotinylated anti- IL-1 beta (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants IL-1β (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat IL-1 beta in serum, plasma & cell culture supernatants. Detection Range: 80 pg/ml - 50,000 pg/ml. 100 assays. BioVision’s rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta. This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 80 pg/ml. Detection Limit: 80 pg/ml – 50000 pg/ml.
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K4798-100
K4798-100? IL-2 Microplate (Item A) coated with anti-human IL-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IL-2) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody IL-2 (Item F), biotinylated anti-human IL-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human IL-2 conc. is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine IL-2 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IL-2 in serum, plasma, urine & cell culture supernatants. Detection Range: 4 pg/ml - 1500 pg/ml. 100 assays. BioVision’s Human IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-2. This assay employs an antibody specific for human IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of IL-2 is typically less than 4 pg/ml. Detection Range: 4 pg/ml – 1500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4799-100
K4799-100? IL-2 Microplate (Item A) coated with anti-mouse IL-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse IL-2) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent, 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. (5x concentrated) ? Detection Antibody IL-2 (Item F), biotinylated anti-mouse IL-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 120x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants IL-2 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse IL-2 in serum, plasma & cell culture supernatants. Detection Range: 1 pg/ml - 40 pg/ml. 100 assays. BioVision’s mouse IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-2. This assay employs an antibody specific for mouse IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF). The minimum detectable dose of IL-2 is typically less than 1 pg/ml. Detection Range: 1 pg/ml – 40 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4800-100
K4800-100? IL-2 Microplate (Item A) coated with anti-rat IL-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant IL-2) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. (5x concentrated) ? Detection Antibody IL-2 (Item F), biotinylated anti-IL-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (rat IL-2 conc. is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants IL-2 (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat IL-2 in serum, plasma & cell culture supernatants. Detection Range: 0.1 ng/ml - 30 ng/ml. 100 assays. BioVision’s rat IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2. This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The minimum detectable dose of IL-2 is typically less than 0.1 ng/ml. Detection Range: 0.1 ng/ml – 30 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K7410-100
K7410-100? Cathepsin D Microplate coated with anti-mouse Ab against Cathepsin D, 96 wells ? Lyophilized recombinant mouse Cathepsin D standard (10 ng/vial) ? Biotinylated anti-mouse Cathepsin D antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA) ? Cell culture supernatantsCathepsin D (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse Cathepsin D in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s mouse Cathepsin D ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form rat specific for Cathepsin D coated on a 96-well plate. Standards (NSO, I21-L410) and test samples are added to the wells and Cathepsin D present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Cathepsin D captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.
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K7338-100
K7338-100? Plate coated with anti-human MCP-1 ? Wash Buffer (20x) (Item B) ? Human MCP-1Standard (Item C) ? Assay Diluent (Item E) (5x) ? Detection Ab MCP-1 (Item F) ? HRP-Streptavidin (Item G) (400x) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? Urine CCL2/MCP-1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CCL2/MCP-1 in serum, plasma, urine & cell culture supernatants. Detection Range: 2 pg/ml – 500 pg/ml. 100 assays.BioVision’s Human MCP-1 (Monocyte chemoattractant protein-1 also called Monocyte chemotactic protein-1) or chemokine ligand 2 (CCL2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MCP-1. This assay employs an antibody specific for human MCP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MCP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of MCP-1 is typically less than 2 pg/ml. Detection Range: 2 pg/ml – 500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4569-100
K4569-100? Plate coated with anti-human GDF-15 ? Wash Buffer (20x) (Item B) ? Human GDF-15Standard (Item C) ? Assay Diluent (Item E) (5x) ? Detection Ab GDF-15 (Item F) ? HRP-Streptavidin (Item G) (500x) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? Urine GDF-15 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human GDF-15 in serum, plasma, cell culture supernatants & urine. Detection Range: 12 pg/ml – 800 pg/ml. 100 assays.BioVision’s Human GDF-15 (Growth Differentiation Factor 15) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDF-15. This assay employs an antibody specific for human GDF-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and GDF-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GDF-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GDF-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. Sensitivity: 2 pg/ml. Detection Range: 2 pg/ml – 800 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K7568-100
K7568-100? Plate coated with anti-mouse Lipocalin-2 ? Wash Buffer (20x) (Item B) ? Mouse Lipocalin-2 Standard (Item C) ? Assay Diluent (Item E) (5x) ? Detection Ab Lipocalin-2 (Item F) ? HRP-Streptavidin (Item G) (500x) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants Lipocalin-2/NGAL (mouse) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of mouse Lipocalin-2/NGAL in serum, plasma & cell culture supernatants. Sensitivity: 0.02 ng/ml. Detection Range: 0.02 ng/ml – 100 ng/ml. 100 assays. BioVision’s Mouse Lipocalin-2 (LCN2)/NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Lipocalin-2. This assay employs an antibody specific for mouse Lipocalin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Lipocalin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Lipocalin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon. Sensitivity: 0.02 ng/ml. Detection Range: 0.02 ng/ml – 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4769-100
K4769-100? Activin A Microplate (Item A) coated with anti-human Activin A, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Activin A) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody Activin A (Item F), biotinylated anti-human Activin A (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Human Activin A ELISA Kit: Colorimetric Assay for Quantitative Determination of human Activin A in Serum, Plasma, Urine & Cell culture supernatants. Detection Range: 15 pg/ml - 5000 pg/ml. 100 assays. BioVision’s Human Activin A ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Activin A. This assay employs an antibody specific for human Activin A coated on a 96-well plate. Standards and samples are pipetted into the wells and Activin A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Activin A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Activin A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Activin A is typically less than 15 pg/ml. Detection Range: 15 pg/ml - 5000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4901-100
K4901-100? Antibody coated 96-well plate (12 x 8-well strips) ? 10X Wash concentrate, 50 ml ? 5X Diluent, 50 ml ? Secondary antibody, 12 ml ? 100X Detector, 150 μl ? Standard, recombinant human adiponectin (64 ng), 1 vial, lyophilized ? QC sample= positive control having 7-11 μg/ml human adiponectin, 1 vial, lyophilized ? Substrate I, 6 ml ? Substrate II, 6 ml ? Stop solution, 12 mlCell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluidsHuman Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in Human Serum, Plasma or Various Tissue or Cell Culture supernatants. 100 Assays.Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This human Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in human serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for human adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-human adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
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K4902-100
K4902-100? Adiponectin antibody-coated plate, 6x16 wells Wash concentrate (10X), 2 x 30 ml Diluent (10X), 2 x 30 ml Secondary Antibody (200X), 60 μl Detector (100X), 150 μl Mouse adiponectin Standard (lyophilized), 16 ng TMB, 12 ml Stop Solution, 12 ml Plate sealers, 2 Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluidsMouse Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in Mouse Serum, Plasma or Various Tissue or Cell Culture supernatants. 100 Assays.Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for mouse adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-mouse adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
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K4903-100
K4903-100? Antibody coated 96-well plate (12x8 well strips) ? 10X Wash concentrate, 50 ml ? 5X Diluent, 50 ml ? Secondary antibody, 12 ml ? 100X Detector (HRP conjugated mouse IgG), 150 μl ? Standard (recombinant rat adiponectin from HEK 293cells), 1 vial, lyophilized (48 ng) ? QC sample= a positive control of rat serum adiponectin, 1 vial, lyophilized (12.67 μg) ? Substrate I, 6 ml ? Substrate II, 6 ml ? Stop solution, 12 mlCell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluidsRat Adiponectin ELISA Assay Kit: Sensitive & Convenient Assay for Quantitative Determination of Adiponectin in rat Serum, Plasma or Various Tissue or Cell Culture supernatants. 100 Assays.Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Rat Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in rat serum, plasma or various tissue or cell culture supernatants. In the assay, polyclonal antibody specific for rat adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and any adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-rat adiponectin monoclonal antibody. With adding HRP conjugated mouse IgG and HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
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