K7116-100
K7116-100? Angiopoietin-2 Microplate (Item A) coated with anti-human Angiopoietin-2, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Angiopoietin-2) ? Assay Diluent (Item E) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent (5X) ? Detection Antibody Angiopoietin-2 (Item F), biotinylated anti-human Angiopoietin-2 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineAngiopoietin-2 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human ANGPT2 in serum, plasma, urine & cell culture supernatants. Detection Range: 10 pg/ml - 3000 pg/ml. 100 assays. BioVision’s Human Angiopoietin-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiopoietin-2. This assay employs an antibody specific for human Angiopoietin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Angiopoietin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, Angiopoietin-1, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13,IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Angiopoietin-2 is typically less than 10 pg/ml. Detection Range: 10 pg/ml – 3000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4755-100
K4755-100? Serum & plasma ? Cell culture supernatants ? Urine Angiostatin (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human Angiostatin in Serum, Plasma, urine & Cell culture supernatants. Detection Limit: less than 20 ng/ml. 100 assays.BioVision’s Human Angiostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Angiostatin. This assay employs an antibody specific for human angiostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and angiostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human angiostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of angiostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of angiostatin is typically less than 20 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4914-100
K4914-100? 1 plate coated with human ANGPTL3 Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial human ANGPTL3 Standard (lyophilized) ? 1 vial human ANGPTL3 QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL3 (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ANGPTL3 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.156–10 ng/ml and Detection limit 75 pg/ml. 100 Assays. The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460-amino acid and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% amino acid sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7 kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of human ANGPTL3 in biological fluids. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL3 in biological fluids. A monoclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples.
More
K4915-100
K4915-100? 1 plate coated with mouse ANGPTL3 Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial mouse ANGPTL3 Standard (lyophilized) ? 1 vial mouse ANGPTL3 QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film) ? 1 plate coated with mouse ANGPTL3 AntibodyCell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL3 (mouse/rat) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of mouse/ rat ANGPTL3 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.016 – 1 ng/ml and Detection limit 15 pg/ml. 100 Assays. The angiopoietins are a family of growth factors that are specific for vascular endothelium. The full-length cDNA encoding angiopoietin-like protein 3 (ANGPTL3) from a human fetal liver/spleen cDNA library has 460-amino acid and the characteristic structure of angiopoietins: a signal peptide, an extended helical domain predicted to form dimeric or trimeric coiled-coils, a short linker peptide, and a globular fibrinogen-like domain (FLD). Human ANGPTL3 shares 76% amino acid sequence identity with mouse Angptl3. Northern blot analysis of human tissues showed a preferential expression of 4 ANGPTL3 transcripts being 4.5, 3.0, 2.8, and 1.7 kb in liver. ANGPTL3 can induce angiogenesis in the rat corneal assay. The FLD alone was sufficient to induce endothelial cell adhesion and in vivo angiogenesis. Microarray analysis showed that mouse hematopoietic stem cell (HSC)-supportive fetal liver CD3-positive cells expressed Angptl2 and Angptl3. The ANGPTL3 ELISA Kit is to be used for the in vitro quantitative determination of mouse/ rat ANGPTL3 in biological fluids. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse or rat ANGPTL3 in biological fluids. A polyclonal antibody specific for ANGPTL3 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL3 is recognized by the addition of a biotinylated polyclonal antibody specific for ANGPTL3 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL3 in the samples. The assay range is 0.016 – 1 ng/ml ANGPTL3/ml. The lowest level of ANGPTL3 that can be detected by this assay is 15 pg/ml.
More
K4916-100
K4916-100? 1 plate coated with human ANGPTL6 Antibody ? Wash Buffer 10X ? Diluent 5X ? Detection Antibody ? Detector 100X (HRP Conjugated anti-rabbit IgG) ? human ANGPTL6 Standard (lyophilized) ? human ANGPTL6 QC sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsANGPTL6 (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ANGPTL6 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 1.56 – 100 ng/ml and Detection limit 1.2 ng/ml. 100 Assays. Seven angiopoietin-like proteins (ANGPTLs) share the characteristic protein structure of the angiopoietin family (ANG), but differ in their inability to bind antiopoietin receptor, Tie-2. ANGPTL6 was originally named angiopoietin-related growth factor (AGF) having an N-terminal coiled-coil-like domain and a C-terminal fibrinogen-like domain, both of which are conserved in ANG. It is a circulating protein secreted by liver that induces angiogenesis by direct effect of epidermal ANGPTL6 on endothelial cells and proliferation of skin cells, and thereby promotes wound healing. 80% of Angptl6 -/- mice died at about embryonic day 13. The surviving null mice developed marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure relative to controls. Conversely, mice with constitutive overexpression of ANGPTL6 showed leanness and increased insulin sensitivity resulting from increased energy expenditure, and were also protected from high-fat diet-induced obesity, insulin resistance, and non-adipose tissue steatosis. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL6 in biological fluids. A monoclonal antibody specific for ANGPTL6 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL6 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL6 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL6 in the samples. The assay range is 1.56 – 100 ng/ml ANGPTL6/ml. The lowest level of ANGPTL6 that can be detected by this assay is 1.2 ng/ml.
More
K4696-100
K4696-100? Plate Coated with ApoE Ab ? Assay Diluent ? Wash Buffer A (10x) ? Wash Buffer B (10x) ? rh ApoE Standard ? Detection Antibody (100x) ? HRP Conjugate (100x) ? TMB Substrate ? Stop Solution ? Plate Sealer ? Serum and plasma ? Cell lysate and cell culture supernatant ? Cerebrospinal Fluid (CSF) Apolipoprotein E (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of human ApoE in serum, plasma, cell lysate & cell culture supernatant, & cerebrospinal fluid. Detection range: 25 ng to 1.6 μg/ml. 100 assays. BioVision’s Human Apolipoprotein E (ApoE) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of human ApoE. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. It is synthesized mainly in liver, while it has also been identified in the brain, kidneys, and spleen. In the nervous system, ApoE is synthesized in non-neuronal cell types, most notably astroglia and microglia, while neurons preferentially express its receptors. Defects in ApoE lead to familial dysbetalipoproteinemia (increased plasma cholesterol and triglycerides). More recently, ApoE has been implicated in several biological processes not directly related to lipoprotein transport, including Alzheimer's disease, immunoregulation, and cognition. The assay employs an antibody specific for human ApoE coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit recognizes ApoE-2, ApoE-3, and ApoE-4 isoforms. Sensitivity of the kit is 25 ng/ml and detection range is from 25 ng to 1.6 μg/ml. Recovery inside this range is between 86 and 111% (average recovery is 102%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
More
K4699-100
K4699-100? Serum and plasma ? Cell lysate and cell culture supernatant ? Cerebrospinal Fluid (CSF) Apolipoprotein E4/Pan-ApoE (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of human ApoE4 in serum, plasma, cell lysate & cell culture supernatant, & cerebrospinal fluid. Detection range: 50 ng to 800 ng/ml. 100 assays. BioVision’s Human Apolipoprotein E4 (ApoE4) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for quantitative measurement of human ApoE4. ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. ApoE exists as three major isoforms, including ApoE2, ApoE3, and ApoE4. Recently, ApoE4 has been implicated in Alzheimer's disease (AD). ApoE4 is the first risk gene identified in Alzheimer's research, and remains the gene with strongest impact. Everyone inherits a copy of ApoE gene from each parent. Those who inherit one copy of ApoE4 have an increased risk of developing AD (about a quarter of the human population). Those who inherit two copies of ApoE4 have an even higher risk (about 2% of humans with up to 10 times higher risk). In addition to raising risk, ApoE4 may tend to make AD symptoms appear at a younger age. The assay employs an antibody specific for human ApoE4 coated on a 96-well plate. Standard and samples are pipetted into wells and ApoE4 present in the sample is bound to the wells by immobilized antibody. Wells are washed and human ApoE4 specific detection antibody is added. After washing away unbound detection antibody, HRP-conjugate is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of bound ApoE4. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 25 ng/ml and detection range is from 50 ng to 800 ng/ml. Recovery inside this range is between 83 and 104% (average recovery is 93%). The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
More
K3599-100
K3599-100? Aromatase Ab-coated plate ? Human Aromatase standard, lyophilized ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from human tissues or cells ? Serum, plasma (EDTA, Heparin) Aromatase (Cytochrome P450 19A1) human ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Aromatase in tissues, cells, plasma, serum or microsomes. Sensitivity: 0.156 ng/ml. Detection Range: 0.156 ng/ml – 10 ng/ml. 100 assays. Aromatase (Cytochrome P450 19A1 or CYP19A1) is a monooxygenase anchored to the Endoplasmic reticulum membrane, its expression being highest in steroidogenic tissue (gonads, breast, adipose tissue, brain, placenta etc.). Aromatase is responsible for conversion of androgens into estrogens and is important in normal sexual development as well as breast, endometrial and other cancers. BioVision’s human Aromatase ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A human Aromatase-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to Aromatase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the human Aromatase captured onto the plate. The concentration of Aromatase in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 0.156 ng/ml – 10 ng/ml. Sensitivity: 0.156 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%. Recovery: Serum (~90%), plasma (EDTA = ~90%, Heparin = ~95%).
More
K3560-100
K3560-100? Aromatase Ab-coated plate ? Rat Aromatase standard, lyophilized ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from rat tissues or cells ? Serum, plasma (EDTA, Heparin) Aromatase (Cytochrome P450 19A1) rat ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of rat Aromatase in tissues, cells, plasma, serum or microsomes. Sensitivity: 1.56 ng/ml. Detection Range: 1.56 ng/ml – 100 ng/ml. 100 assays. Aromatase (Cytochrome P450 19A1 or CYP19A1) is a monooxygenase anchored to the Endoplasmic reticulum membrane, its expression being highest in steroidogenic tissue (gonads, breast, adipose tissue, brain, placenta etc.). Aromatase is responsible for conversion of androgens into estrogens and is important in normal sexual development as well as breast, endometrial and other cancers. BioVision’s rat Aromatase ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat Aromatase-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to Aromatase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the rat Aromatase captured onto the plate. The concentration of Aromatase in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 1.56 ng/ml – 100 ng/ml. Sensitivity: 1. 56 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%. Recovery: Serum (~90%), plasma (EDTA = ~90%, Heparin = ~85%).
More
K4744-100
K4744-100? BMP-2 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-2. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-2. ? Assay Diluent A (Item D): 30 ml of diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-2 (Item F): 2 vial of biotinylated anti human BMP-2 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 200x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-2 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-2 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 45 pg/ml. 100 assays.BioVision’s Human BMP-2 (Bone morphogenetic proteins-2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-2. This assay employs an antibody specific for human BMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-2 is typically less than 45 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. In this ELISA formate, the pair antibody shows less than 1% cross-reactivity with rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6 or rhBMP-7.
More
K4745-100
K4745-100? BMP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-4. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-4. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-4 (Item F): 2 vial of biotinylated anti human BMP-4 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 80x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-4 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-4 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 15 pg/ml. 100 assays.BioVision’s Human BMP-4 (Bone morphogenetic proteins-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-4. This assay employs an antibody specific for human BMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4746-100
K4746-100? BMP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-4. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-4. ? Sample Diluent Buffer (Item D): 10 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissue lysate) diluent. ? Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Detection Antibody (Item F) & HRP-Streptavidin Concentrate (Item G) diluent. ? Detection Antibody BMP-4 (Item F): 2 vial of biotinylated anti human BMP-4 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 80x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Cell lysate buffer (Item J): 5 ml 2X cell lysate buffer. ? Cell lysate & tissue lysateBMP-4 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-4 in cell & tissue lysates. Detection Limit: less than 15 pg/ml. 100 assays.BioVision’s Human BMP-4 (Bone morphogenetic proteins-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-4. This assay employs an antibody specific for human BMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4747-100
K4747-100? BMP-5 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-5. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-5. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-5 (Item F): 2 vial of biotinylated anti human BMP-5 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 300x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma (BMP-5 concentration in human normal serum/plasma is pretty low, it may not be detected in this assay) ? Cell culture supernatants ? Urine BMP-5 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-5 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 0.25 ng/ml. 100 assays.BioVision’s Human BMP-5 (Bone morphogenetic proteins-5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-5. This assay employs an antibody specific for human BMP-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-5 is typically less than 0.25 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4748-100
K4748-100? BMP-6 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-6. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-6. ? Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (serum/plasma/cell culture medium/urine) & reagent diluent. ? Detection Antibody BMP-6 (Item F): 2 vial of biotinylated anti-human BMP-6 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 1,500x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-6 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-6 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 150 pg/ml. 100 assays.BioVision’s Human BMP-6 (Bone morphogenetic proteins-6) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-6. This assay employs an antibody specific for human BMP-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-6 is typically less than 150 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4749-100
K4749-100? BMP-7 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-7. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human BMP-7. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody BMP-7 (Item F): 2 vial of biotinylated anti human BMP-7 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 400x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma ? Cell culture supernatants ? Urine BMP-7 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human BMP-7 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 10 pg/ml. 100 assays.BioVision’s Human BMP-7 (Bone morphogenetic proteins-7) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-7. This assay employs an antibody specific for human BMP-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-7 is typically less than 10 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
|
|