K7422-100
K7422-100? Plate coated with T3 Ab ? T3 Standard ? Assay Diluent ? T3 Enzyme Conjugate Conc. ? Wash Concentrate (20X) ? TMB Substrate ? Stop SolutionSerum or plasma Triiodothyronine (T3) (Mouse/Rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of mouse/rat Triiodothyronine in serum or plasma. Sensitivity: 0.186 ng/ml. 100 assays. Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s mouse/rat Triiodothyronine (T3) kit is a solid phase competitive ELISA Kit. The samples, and T3 enzyme conjugate are added to the wells coated with anti-T3 polyclonal antibody. T3 in the sample competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
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K4917-100
K4917-100? 1 plate coated with human Vaspin Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Conjugated anti-rabbit IgG) ? 1 vial human Vaspin Standard (lyophilized) ? 1 vial human Vaspin QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsVaspin (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Vaspin in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.016 – 1 ng/ml and Detection limit 12 pg/ml. 100 Assays. Vaspin, designated as visceral adipose tissue-derived serpin, is a serpin whose expression is restricted to visceral adipose tissue. Vaspin seems to retain the serpin signature conformation fold consisting of three β-sheets and nine α-helices as well as a reactive loop site that interacts with its cognate serine protease unidentified hitherto. Vaspin is remarkably upregulated at high-fat high-sucrose (HFHS) diet and at multiple metabolic dysfunctions like obesity or insulin resistance. Administration of thiazolidinedione (TZD) caused a significant induction of serum vaspin in mice. These facts imply that upregulation of vaspin may be a compensatory response to antagonize the action of other unknown proteases upregulated in obesity or insulin resistance. Indeed, administration of recombinant vaspin to obese mice fed with HFHS chow improved glucose tolerance and insulin sensitivity. Vaspin seems to directly act on white adipose tissue. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human vaspin in biological fluids. A monoclonal antibody specific for vaspin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, vaspin is recognized by the addition of a purified polyclonal antibody specific for vaspin (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of vaspin in the samples. The assay range is 0.016 – 1 ng/ml Vaspin/ml. The lowest level of vaspin that can be detected by this assay is 12 pg/ml.
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K7211-100
K7211-100? VCAM-1 Microplate coated with anti-human monoclonal Ab against VCAM-1, 96 wells ? Lyophilized recombinant human VCAM-1 standard (10 ng/vial) ? Biotinylated anti- human VCAM-1antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatantsVCAM-1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human VCAM-1 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s human VCAM-1 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for human VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human VCAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.
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K7212-100
K7212-100? VCAM-1 Microplate coated with anti-mouse monoclonal Ab against VCAM-1, 96 wells ? Lyophilized recombinant mouse VCAM-1 standard (10 ng/vial) ? Biotinylated anti- mouse VCAM-1antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatantsVCAM-1 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse VCAM-1 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s mouse vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for mouse VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VCAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 5 pg/ml.
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K5363-100
K5363-100? Plate coated with anti-human VEGF antibody, 96-wells ? Human VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-human VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatants ? UrineVEGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 31.2 pg/ml – 2000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. VEGF mediates progression of Diabetic retinopathy as well as cancer metastasis. BioVision’s human VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 31.2 pg/ml – 2000 pg/ml. Sensitivity: < 1 pg/ml.
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K5364-100
K5364-100? Plate coated with anti-mouse VEGF antibody, 96-wells ? Mouse VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-mouse VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatants ? UrineVEGF (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of murine VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 15.6 pg/ml – 1000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s mouse VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VEGF captured onto the plate. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 2 pg/ml.
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K5365-100
K5365-100? Plate coated with anti-rat VEGF antibody, 96-wells ? Rat VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-rat VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatants ? UrineVEGF (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 15.6 pg/ml – 1000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s rat VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
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K4806-100
K4806-100? 96 wells coated with anti-human Vit. D3 antibody, 1 Microplate ? Human Vit. D3 standard (0.9 ng/μl) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Sealed Bag ? Plate cover ? Serum & plasma (EDTA/Citrate) ? Urine ? Cell culture medium, Tissue homogenates and cell lysatesVitamin D3 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Vitamin D3 (cholecalciferol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:200 – 800 ug/L. 100 assays. Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. BioVision’s human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 – 800 μg/L.
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K3381-100
K3381-100? 96 wells coated with anti-human β-catenin antibody, 1 Microplate ? Human β-catenin standard (18 ng/ml) ? HRP-conjugate reagent ? Standard Diluent ? Sample Diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Sealed Bag ? Plate cover ? Serum and other biological fluids ? Cell culture medium, tissue homogenates and cell lysates β-catenin (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human β-catenin in serum, biological fluids, cell culture media and cell/tissue lysates. Detection Range: 0.156 – 10 ng/ml. 100 assays. β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s human β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 0.156 – 10 ng/ml.
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K3382-100
K3382-100? 96 wells coated with anti-mouse β-catenin antibody, 1 Microplate with 2 adhesive strips ? Mouse β-catenin standard (270 ng/L) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Serum & plasma (EDTA/Citrate), urine and other biological fluids ? Cell culture medium, tissue homogenates and cell lysatesβ-catenin (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse β-catenin in serum, plasma, urine, biological fluids, cell culture media and cell/tissue lysates. Detection Range: 3 – 180 ng/L. 100 assays. β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s mouse β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Mouse β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 3 – 180 ng/L.
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K3383-100
K3383-100? 96 wells coated with anti-rat β-catenin antibody, 1 Microplate with 2 adhesive strips ? Rat β-catenin standard (360 ng/L) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Serum & plasma (EDTA/Citrate), urine and other biological fluids ? Cell culture medium, tissue homogenates and cell lysatesβ-catenin (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat β-catenin in serum, plasma, urine, biological fluids, cell culture media and cell/tissue lysates. Detection Range: 5 – 240 ng/L. 100 assays. β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s rat β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 5 – 240 ng/L.
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K5351-100
K5351-100? Plate coated with TPO recombinant Ag ? Sample Diluent ? Calibrator ? Positive Control ? Negative Control ? Enzyme Conjugate ? Wash Concentrate (20X) ? TMB Substrate ? Stop Solution Serum or plasmaThyroid Peroxidase [TPO] IgG (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of TPO IgG in human serum or plasma. 100 assays. Thyroid peroxidase (TPO) is the major autoantigen (933 amino acid residue) in the thyroid microsomal antigen (TMA) particle. Detection of TPO antibodies is strong evidence against a goiter or non-autoimmune causes of hypothyroidism. The annual risk for the development of hypothyroidism is 3% to 4% per year if TPO antibodies are present and TSH is elevated. TPO antibodies are present in 8-9% normal controls, 57-74% patients with Graves disease, 99-100% of Hashimoto disease or idiopathic myxedema, 19% with differentiated thyroid cancer, and 11% of those with other miscellaneous non-autoimmune thyroid diseases. The prevalence of positive TPO antibodies is higher in elderly (mean age 80 years) women (10%) compared to elderly men (2%).Autoantibody concentration in centenarians also decreases. Studies of TPO epitopes in each domain, A and B, and detection of their specific autoantibodies suggest that the epitope-specific TPO antibodies ratio (A/B) does not change over time in individual patients and that TPO epitope autoantibody patterns may be inherited. In BioVision’s TPO IgG ELISA Kit, sample is added to the wells coated with purified TPO recombinant antigen. TPO IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
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K7411-100
K7411-100? Microplate coated with TSH MAb, 96 wells ? TSH Standard: (0.5 ml) (ready to use) ? TSH Enzyme Conjugate (ready to use) ? Wash Concentrate (20X) ? TMB Substrate (ready to use) ? Stop Solution (ready to use) ? SerumThyroid Stimulating Hormone (human) ELISA Kit: Simple & convenient colorimetric assay for quantitative measurement of human TSH in serum. Sensitivity: 0.05 μIU/ml. 100 assays. Thyroid Stimulating Hormone (TSH) is a glycoprotein hormone secreted by the pituitary gland and regulates the synthesis/ release of T3 and T4 by thyroid gland. Increased serum TSH is an early and sensitive indicator of decreased thyroid reserve and overt primary hypothyroidism. Decrease TSH levels is an indicator of TSH-independent hyperthyroidism (Graves disease). BioVision’s human TSH kit is a solid phase sandwich ELISA Kit. The samples, and anti-TSH-HRP conjugate are added to the wells coated with monoclonal antibody to TSH beta subunit. TSH in the sample binds to anti-TSH MAb on the well and the anti-TSH detection antibody then binds to TSH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of TSH in the samples. A standard curve is prepared relating color intensity to the concentration of the TSH. The sensitivity of this ELISA test is 0.05μIU/ml.
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K4918-100
K4918-100? 1 plate coated with human ACE2 Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial human ACE2 Standard (lyophilized) ? 1 vial human ACE2 QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsACE2 (human) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human ACE2 in Urine, Cell Culture Supernatant etc.. Assay Range 0.391–25 ng/ml and Detection limit 293 pg/ml.100 Assays. By EST database searching for sequences showing homology to the zinc metalloprotease angiotensin-I converting enzyme, a full-length ACE2 cDNA, originally called ACEH, was isolated, which encoded a deduced 805-amino acid protein that shares approximately 40% identity with the N- and C-terminal domains of ACE. ACE2 contains a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. Northern blot analysis detected high expression of ACE2 in kidney, testis, and heart, and moderate expression in colon, small intestine, and ovary. The soluble, truncated form of ACE2 lacks the transmembrane and cytosolic domains in CHO cells and is glycosylated protein that was able to cleave angiotensin I and angiotensin II , but not bradykinin. ACE converts angiotensin I to angiotensin II, which has 8 amino acids, ACE2 converts angiotensin I to angiotensin 1-9, which has 9 amino acids. This can then further be converted by ACE to a shorter peptide, angiotensin 1-7, which is a blood vessel dilator. Using ACE2 null mice, Crackower et al. showed that ACE2 was critically involved in a cardiac contractility. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ACE2 in biological fluids. A polyclonal antibody specific for ACE2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ACE2 is recognized by the addition of a biotinylated polyclonal antibody specific for ACE2 (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ACE2 in the samples. The assay range is 0.391–25 ng/ml ACE2/ml. The lowest level of ACE2 that can be detected by this assay is 293 pg/ml.
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24-MYOHU-E01
Myoglobin ELISA Sensitivity:6.25 ng/mL Sample Types:Serum, Urine Sample Sizes:10 μL Range:0 - 250 ng/mL
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