K4922-100
K4922-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Lysis Buffer (10X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Mouse FTO Standard (lyophilized, 20 ng) ? Mouse FTO QC Sample (lyophilized) ? TMB Substrate Solution ? Stop Solution ? Plate SealersCell lysatesFTO (mouse intracellular) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Mouse FTO in cells. Assay Range 0.156 – 10 ng/ml and Detection limit 20 pg/ml. 100 Assays. FTO, Fat mass-and obesity-associated gene, was discovered as a responsible gene causing the mouse ‘fused toes’ mutation. The predicted 502-amino acid Fto protein has a calculated molecular mass of 58 kD and contains an N-terminal bipartite nuclear localization signal. RT-PCR detected Fto expression throughout mouse embryonic development and in all adult mouse tissues examined except heart and skin. Bioinformatics analysis indicates that FTO shares sequence motifs with iron- and 2-oxoglutarate (2OG)-dependent oxygenases. Recombinant murine Fto catalyzes the iron- and 2OG-dependent demethylation of 3-methylthymine in single-stranded DNA, with concomitant production of succinate, formaldehyde, and carbon dioxide. In wildtype mice, Fto mRNA is most abundant in the brain, particularly in hypothalamic nuclei, and are regulated by feeding and fasting. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of FTO in the samples. This ELISA is specific for the measurement of natural and recombinant mouse FTO. It does cross-react with mouse adiponectin, mouse RBP4, mouse Nampt, mouse vaspin, mouse progranulin, mouse resistin, mouse clusterin, mouse GPX3, mouse CD137, rat adiponectin, rat Nampt, human adiponectin, human Nampt, human progranulin. The assay range is 0.156 – 10 ng FTO/ml and a detection limit of 20 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
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K4772-100
K4772-100? GM-CSF Microplate (Item A) coated with anti-human GM-CSF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human GM-CSF) ? Assay Diluent (Item E) for Standard/Sample (serum/plasma/ cell culture medium/Urine) diluent (5x con.) ? Detection Antibody GM-CSF (Item F), biotinylated anti-human GM-CSF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine GM-CSF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human GM-CSF in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 2 pg/ml - 500 pg/ml. 100 assays. BioVision’s human GM-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GM-CSF. This assay employs an antibody specific for human GM-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of GM-CSF is typically less than 2 pg/ml. Detection Range: 2 pg/ml - 500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4279-100
K4279-100? 96 wells coated with anti-human monoclonal Granzyme B antibody, 1 Microplate ? Lyophilized recombinant human Granzyme B standard (10 ng/vial) ? Biotinylated anti- human Granzyme B antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (heparin, EDTA) ? Cell culture supernatants Granzyme B (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Granzyme B in serum, plasma & cell culture supernatants.Sensitivity: < 10 pg/ml. 100 assays. Granzyme B is a serine protease expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and functions in cell-mediated immunity. BioVision’s human Granzyme B ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human Granzyme B is coated on a 96-well plate. Standards and test samples are added to the wells and Granzyme B present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Granzyme B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Granzyme B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml.
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K7412-100
K7412-100? Microplate coated with hGH MAb, 96 wells ? hGH Standard: (0.7 ml) (ready to use) ? hGH Enzyme Conjugate (ready to use) ? Wash Concentrate (20X) ? TMB Substrate (ready to use) ? Stop Solution (ready to use) ? Serum or plasma Growth hormone (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human growth hormone in serum or plasma. 100 assays. Human GH (Growth Hormone) has profound effects on tissue growth & metabolism. Hyposecretion of hGH becomes apparent in infants a few months after birth & may result in dwarfism. On the contrary, hypersercretion of hGH results in gigantism. BioVision’s human GH kit is a solid phase sandwich ELISA Kit. The samples, and anti-hGH-HRP conjugate are added to the wells coated with hGH monoclonal antibody. hGH in the sample binds to anti-hGH MAb on the well and the anti-HGH second antibody then binds to hGH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of hGH in the samples. A standard curve is prepared relating color intensity to the concentration of the hGH. Expected value of hGH in adults (< 10 ng/ml), & in children (< 20 ng/ml).
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K926-100
K926-100? βIG-H3 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human βIG-H3. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials of recombinant human βIG-H3. ? Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody βIG-H3 (Item F): 2 vial of biotinylated anti-human βIG-H3 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl 300x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in buffer solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. Serum, Plasma, Cell culture supernatants and urineHuman βIG-H3 ELISA Kit: Measure human βIG-H3 in Serum, Plasma, Cell culture Supernatants, Urine etc. Direct, Convenient Colorimetric Method. 100 assays.BioVision’s Human βIG-H3 (also known as Kerato-epithelin, RGDCAP) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human βIG-H3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human βIG-H3 coated on a 96-well plate. Standards and samples are pipetted into the wells and βIG-H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human βIG-H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of βIG-H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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K4777-100
K4777-100? Leptin Microplate (Item A) coated with anti-human Leptin, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Leptin) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody Leptin (Item F), biotinylated anti-human Leptin (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 160x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Leptin (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human leptin in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 2 pg/ml - 400 pg/ml. 100 assays. BioVision’s Human Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Leptin. This assay employs an antibody specific for human Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of Leptin is typically less than 2 pg/ml. Detection Range: 2 pg/ml - 400 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4778-100
K4778-100? LIF Microplate (Item A) coated with anti-human LIF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human LIF) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody LIF (Item F), biotinylated anti-human LIF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 320x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Lif (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Lif in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 15 pg/ml - 6000 pg/ml. 100 assays. BioVision’s Human LIF (Leukocyte inhibitory factor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LIF. This assay employs an antibody specific for human LIF coated on a 96-well plate. Standards and samples are pipetted into the wells and LIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human LIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of LIF is typically less than 15 pg/ml. Detection Range: 15 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K7426-100
K7426-100? Plate coated with LH MAb ? LH Standard ? LH Enzyme Conjugate ? Wash Concentrate (20X) ? TMB Substrate ? Stop Solution Luteinizing Hormone (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human LH in serum. 100 assays. Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), that is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is glycoprotein with a molecular weight of approximately 30,000 Dalton. LH stimulates ovulation and ovarian steroid production in the female. In the male, LH controls Leydig cell secretion of testosterone. LH is elevated in Luteal phase of menstrual cycle, primary hypogonadism, Gonadotropin-secreting pituitary tumors and menopause. LH is deceased in hypothalamic Gn-RH deficiency, pituitary LH deficiency and ectopic steroid production. BioVision’s human LH kit is a solid phase direct sandwich ELISA Kit. The samples, and anti-LH-HRP conjugate are added to the wells coated with monoclonal antibody to LH beta subunit. LH in the sample binds to anti-LH MAb on the well and the anti-LH second antibody then binds to LH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of LH in the samples. A standard curve is prepared relating color intensity to the concentration of the LH.
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61-LGPHU-E01
sLIF-R gp190 ELISA (Leukemia Inhibitory Factor Receptor gp190 ELISA) Sensitivity:0.05 ng/mL Sample Types:Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum Sample Sizes:25 μL Range:0.08 - 5.0 ng/mL
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K4754-100
K4754-100? Serum & plasma ? Cell culture supernatants ? Urine TACE (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of human TACE in Serum, Plasma, urine & Cell culture supernatants. Detection Limit: less than 70 pg/ml. 100 assays.BioVision’s Human TACE (TNF-alpha converting enzyme) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TACE. This assay employs an antibody specific for human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TACE is typically less than 70 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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61-LSEHU-E01
sL-selectin ELISA Sensitivity:0.2 ng/mL Sample Types:Cell Culture, EDTA Plasma, Heparin Plasma, Serum Sample Sizes:50 μL Range:0.4 - 25.0 ng/mL
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61-PECHU-E01
sPECAM-1 ELISA (Platelet Endothelial Cell Adhesion Molecule ELISA) Sensitivity:0.06 ng/mL Sample Types:Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine Sample Sizes:10 μL Range:0.47 - 30.0 ng/mL
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61-PSEHU-E01
sP-selectin ELISA Sensitivity:0.20 ng/mL Sample Types:Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum Sample Sizes:10 μL Range:0.63 - 40.00 ng/mL
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61-PSGHU-E01
sPSGL-1 ELISA (P-Selectin Glycoprotein Ligand-1 ELISA) Sensitivity:1.0 U/mL Sample Types:Cell Culture, Serum Sample Sizes:100 μL Range:1.6 - 50 U/mL
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30-1016
sRANKL ELISA (Total) Sensitivity:1.5 pg/mL Sample Types:Cell Culture, Plasma, Serum Sample Sizes:10 μL Range:0 - 60 pg/mL
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