K7427-100
K7427-100? Plate coated with CA19-9 MAb ? CA19-9 Standard ? CA19-9 Assay Buffer ? Enzyme Conjugate Conc. (12x) ? CA19-9 Conjugate Diluent ? Wash Concentrate (20X) ? TMB Substrate ? Stop Solution SerumCA19-9 (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Cancer Antigen 19-9 (CA19-9) in serum. 100 assays. CA19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of CA19-9 in tissues is consistent with the quantitative determination of higher CA19-9 concentrations in cancer than in normal or inflamed tissues. Recently reports indicates that the serum CA19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA, elevated CA19-9 is suggestive of gallbladder neoplasm in the setting of inflammatory gallbladder disease. It has been shown that a persistent elevation in serumCA19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum CA 19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA 19-9 value may be indicative of a favorable prognosis and good response to treatment. The CA19-9 ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA19-9 molecule for immobilization (on the microtiter wells). Another CA 19-9 monoclonal antibody conjugated to horseradish peroxidase (HRP) is in the antibody-enzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA19-9 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate incubation steps at 37°C for 90 min., the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 min., resulting in development of blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CA19-9 is directly proportional to the color intensity of the test sample.
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K4803-100
K4803-100? CA-125 Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CA-125 Standard (Item C) ? Assay Diluent (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (800x ) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCancer Antigen 125 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CA-125 in serum, plasma, urine & cell culture supernatants. Detection Range: 0.6 U/ml - 400 U/ml. 100 assays. BioVision’s Human Cancer Antigen 125 (CA-125) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-125. This assay employs an antibody specific for human CA-125 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA-125 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA-125 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA-125 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CA-125 is typically less than 0.6 U/ml. Detection Range: 0.6 U/ml – 400 U/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4804-100
K4804-100? CA15-3 Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CA15-3 Standard (Item C) ? Assay Diluent A (Item D) ? Assay Diluent B (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (200x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCancer Antigen 15-3 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CA15-3 in serum, plasma, urine & cell culture supernatants. Detection Range: 4 mU/ml - 1000 mU/ml. 100 assays. BioVision’s Human Cancer antigen 15-3 (CA15-3) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA15-3. This assay employs an antibody specific for human CA15-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA15-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA15-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA15-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CA15-3 is typically less than 4 mU/ml. Detection Range: 4 mU/ml – 1000 mU/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4805-100
K4805-100? CEA Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human CEA Standard (Item C) ? Assay Diluent A (Item D) ? Assay Diluent B (5x) (Item E) ? Detection Antibody (Item F) ? HRP-Streptavidin (1000x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? UrineCarcinoembryonic Antigen (CEA) (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CEA in serum, plasma, urine & cell culture supernatants. Detection Range: 0.2 ng/ml - 250g/ml. 100 assays. BioVision’s Human Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CEA is typically less than 200 pg/ml. Detection Range: 0.2 ng/ml – 250 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K3072-100
K3072-100? CD40 Microplate (Item A) coated with anti-human CD40, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human CD40) ? Assay Diluent D (Item K) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent. 5x concentrated ? Assay Diluent B (Item E) for detection antibody & HRP-Streptavidin diluent. 5x concentrated ? Detection Antibody CD40 (Item F), biotinylated anti-human CD40 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineCD40 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CD40 in serum, plasma, urine & cell culture supernatants. Detection Range: 50 pg/ml - 40000 pg/ml. 100 assays. BioVision’s Human CD40 (TNFRSF5) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40. This assay employs an antibody specific for human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin (OB), MCP-1, MDC, MIP-1α, MIP-1 β, MIP-1δ, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CD40 is typically less than 50 pg/ml. Detection Range: 50 pg/ml – 40000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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30-9411
A Proliferation-Inducing Ligand ELISA (APRIL ELISA) Sensitivity:15.6 ng/mL Sample Types:Cell Culture, Plasma Sample Sizes:100 μL Range:15.6 - 1000 ng/mL
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22-ALS-E35
Acid Labile Subunit ELISA Sensitivity:0.23 mU/mL Sample Types:Serum Sample Sizes:5 μL Range:1.5 - 40 mU/mL
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32-5881
AcSDKP ELISA (N-acetyl-Ser-Asp-Lys-Pro) Sensitivity:0.19 nM Sample Types:Plasma, Serum, Urine Sample Sizes:150 μL Range:0.19 - 25 nM
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62-CATHU-E01
Active Dephosphorylated Beta-Catenin ELISA (S33/S37/T41) Sensitivity: Sample Types:Cell Culture, Tissue Culture Sample Sizes:100 μL Range:
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32-5106
Acylated Ghrelin ELISA Sensitivity:0.8 pg/mL Sample Types:Plasma Sample Sizes:100 μL Range:1.95 - 250 pg/mL
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20-AVAHU-E01
Adenovirus IgA ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos./ neg. / cut off control
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20-AVGHU-E01
Adenovirus IgG ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos./ neg. / cut off control
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20-AVMHU-E01
Adenovirus IgM ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos. / neg. control
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32-5181
Adipocyte Fatty Acid Binding Protein ELISA (AFABP ELISA) Sensitivity:0.1 ng/mL Sample Types:Cell Culture, Plasma, Serum Sample Sizes:20 μL Range:0.5 - 25 ng/mL
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30-7860
ADMA Xpress ELISA (Asymmetric Dimethylarginine ELISA) Sensitivity:0.1 μM Sample Types:EDTA Plasma, Serum Sample Sizes:25 μL Range:0.1 μM -2 μM
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