K7871-100
K7871-100? Human CPR Ab coated plate ? Human CPR standard ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from human tissues or cellsCytochrome P450 Reductase (CPR) Human ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human CPR in tissues, cells or microsomes. Sensitivity: 0.156 ng/ml. Detection Range: 0.156 ng/ml – 10 ng/ml. 100 assays. NADPH-cytochrome P450 reductase (CPR, EC 1.6.2.4) is a ~78 kDa membrane-bound flavoenzyme that catalyzes the transfer of electrons from NADPH to members of the cytochrome P450 monooxidase (CYP) enzyme family in the endoplasmic reticulum. As CPR is required for the function of all CYP isozymes, it plays a critical role in the metabolism of drugs, organic pollutants and other xenobiotic compounds, in addition to its role in biosynthesis of certain vitamins and steroid hormones. BioVision’s human CPR ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A human CPR-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to CPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain CPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the human CPR captured onto the plate. The concentration of CPR in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 0.156 ng/ml – 10 ng/ml. Sensitivity: 0.156 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%.
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K7872-100
K7872-100? Rat CPR Ab coated plate ? Rat CPR standard ? Detection Reagent A ? Detection Reagent B ? Standard diluent ? Wash Buffer (30X) ? Assay diluent A ? Assay diluent B ? TMB ? Stop solution ? Plate Sealers ? Tissue or cell lysates ? Microsomes from rat tissues or cellsCytochrome P450 Reductase (CPR) Rat ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of rat CPR in tissues, cells or microsomes. Sensitivity: 0.312 ng/ml. Detection Range: 0.312 ng/ml – 20 ng/ml. 100 assays. NADPH-cytochrome P450 reductase (CPR, EC 1.6.2.4) is a ~78 kDa membrane-bound flavoenzyme that catalyzes the transfer of electrons from NADPH to members of the cytochrome P450 monooxidase (CYP) enzyme family in the endoplasmic reticulum. As CPR is required for the function of all CYP isozymes, it plays a critical role in the metabolism of drugs, organic pollutants and other xenobiotic compounds, in addition to its role in biosynthesis of certain vitamins and steroid hormones. BioVision’s rat CPR ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat CPR-specific antibody is coated on the 96-well plate. Standards or samples are then added to the appropriate wells with a biotin-conjugated antibody specific to CPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. After TMB substrate solution is added, only those wells that contain CPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color to form a blue product. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change from blue to yellow is measured spectrophotometrically at 450nm . The density of yellow color is proportional to the rat CPR captured onto the plate. The concentration of CPR in the samples is then determined by comparing the O.D. of the samples to the standard curve. This ELISA kit shows no detectable cross-reactivity with related isoforms. Detection Range: 0.312 ng/ml – 20 ng/ml. Sensitivity: 0.312 ng/ml. Assay Precision: Intra-assay CV<10% and Inter-assay CV<12%.
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K7428-100
K7428-100? Plate coated with Goat anti-mouse IgG ? Standard set (0.25 ml) ? DHEA-S Enzyme Reagent ? Anti-DHEA-S Ab Reagent ? Wash Concentrate (20X) ? TMB Substrate ? Stop Solution Serum and plasmaDHEA-S (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Dehydroepiandrosterone sulfate (DHEA-S) in serum & plasma. 100 assays.
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K4770-100
K4770-100? DPP IV Ab coated Microplate (Item A), 96 wells ? Wash Concentrate (20x) (Item B) ? Mouse DPP IV Standards (Item C) ? Assay Diluent C (Item L) ? Assay Diluent B (5x) (Item E) ? Biotinylated anti-mouse DPP IV Ab (Item F) ? HRP-Streptavidin (200x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & Plasma ? Cell culture supernatants DPP4 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse DPP 4 in Serum, Plasma & Cell culture supernatants. Detection Range: 0.45 ng/ml - 100 ng/ml. 100 assays. BioVision’s Mouse DPP IV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DPP IV. This assay employs an antibody specific for DPP IV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP IV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPP IV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP IV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of DPP IV is typically less than 0.45 ng/ml. Detection Range: 0.45 ng/ml - 100 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
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K4801-100
K4801-100? CD26 Microplate (Item A) coated with anti-human CD26, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human CD26) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative. ? Assay Diluent B (Item E) for Standard/Sample (cell culture supernatant/urine) (5x concentrated) ? Detection Antibody CD26 (Item F), biotinylated anti-human CD26 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 500x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & Plasma ? Cell culture supernatants ? urine DPP IV/CD26 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CD26 in serum, plasma, urine & cell culture supernatants. Detection Range: 25 pg/ml - 8,000 pg/ml. 100 assays. BioVision’s human DPP IV (Dipeptidyl peptidase-4) or CD26 (cluster of differentiation 26) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD26. This assay employs an antibody specific for CD26 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD26 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD26 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD26 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of CD26 is typically less than 25 pg/ml. Detection Range: 25 pg/ml – 8,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
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K4022-100
K4022-100? EGF Microplate coated with anti-human monoclonal Ab against EGF, 96 wells ? Lyophilized recombinant human EGF standard (10 ng/vial) ? Biotinylated anti- human EGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (heparin, EDTA, citrate) ? Cell culture supernatants ? Urine EGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human EGF in serum, plasma, urine & cell culture supernatants. Detection Range: 4.7-300 pg/ml. 100 assays.BioVision’s human EGF (Epidermal Growth Factor) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for EGF coated on a 96-well plate. Standards (E. Coli, N971-R1023) and test samples are added to the wells and EGF present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human EGF captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 4.7 pg/ml – 300 pg/ml. Sensitivity: < 1 pg/ml.
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K4023-100
K4023-100? EGF Microplate coated with anti-mouse monoclonal Ab against EGF, 96 wells ? Lyophilized recombinant mouse EGF standard (10 ng/vial) ? Biotinylated anti-mouse EGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA) ? Cell culture supernatants ? Urine ? Tissue homogenatesEGF (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse EGF in serum, plasma, urine, tissue homogenates & cell culture supernatants. Detection Range: 15.6-1000 pg/ml. 100 assays.BioVision’s mouse EGF (Epidermal Growth Factor) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from rat specific for EGF coated on a 96-well plate. Standards (E. Coli, N977-R1029) and test samples are added to the wells and EGF present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse EGF captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
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K4024-100
K4024-100? EGF Microplate coated with anti-rat monoclonal Ab against EGF, 96 wells ? Lyophilized recombinant rat EGF standard (10 ng/vial) ? Biotinylated anti-rat EGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA) ? Cell culture supernatants ? Urine ? Tissue homogenatesEGF (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat EGF in serum, plasma, urine, tissue homogenates & cell culture supernatants. Detection Range: 7.8-500 pg/ml. 100 assays.BioVision’s rat EGF (Epidermal Growth Factor) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for EGF coated on a 96-well plate. Standards (E. Coli, N974-R1026) and test samples are added to the wells and EGF present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat EGF captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 7.8 pg/ml – 500 pg/ml. Sensitivity: < 1 pg/ml.
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K7429-100
K7429-100? Plate coated with mouse MAb to ET-1 ? ET-1 Ab Concentrate ? Antibody Diluent ? Assay Buffer 17 ? Wash Buffer Concentrate ? ET-1 Standard (1000 pg/ml) ? TMB Substrate ? Stop Solution 2 (1 N HCl in H20) ? Plate Sealer Serum, plasma, culture supernatantsEndothelin-1 (ET-1) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human, mouse, rat, cow, dog, pig and rabbit ET-1. Sensitivity: 0.4 pg/ml (range: 0.78-100 pg/ml). 100 assays. BioVision’s Endothelin-1 (ET-1) ELISA kit is a competitive immunoassay for the quantitative determination of Endothelin-1 in biological fluids. ET-1 has identical amino acid sequence for human, mouse, rat, cow, dog, pig, and rabbit. The basal circulating level of ET-1 is reported to be < 1 to 3pg/mL but is known to be elevated in atrial and pulmonary hypertensions, atherosclerosis, congestive heart failure, cancer and variably related to lung diseases such as COPD and asthma. Samples and standards are added to wells coated with a monoclonal antibody specific for ET-1 and incubated. The plate is washed, leaving only bound ET-1 on the plate. A solution of HRP labeled monoclonal antibody to ET-1 is then added. This binds the ET-1 captured on the plate. The plate is then incubated. The plate is washed to remove excess HRP labeled antibody. TMB Substrate solution is added. The substrate generates a blue color when catalyzed by the HRP. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of ET-1 in the sample. Sensitivity: 0.4 pg/ml (range: 0.78-100 pg/ml). The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Cross Reactivity: ET-1: 100%, ET-2: 21%, ET-3: 3.6%, human Big ET-1: <0.1%, rat Big ET-1: <0.1%, human Big ET-2: <0.1%, human Big ET-3 amide: <0.1%.
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K3829-100
K3829-100? 96 wells coated with anti-human Estradiol antibody, 1 Microplate with 2 adhesive strips ? Human Estradiol standard (72 pmol/L) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Serum & plasma (EDTA/Citrate), urine ? Cell culture medium, tissue homogenates and cell lysates Estradiol E2 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Estradiol (E2 or 17β-estradiol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:2 – 50 pmol/L. 100 assays. Estradiol (E2 or 17β-estradiol) is the predominant female reproductive hormone secreted by the ovaries. Smaller amounts of estradiol are also produced by the adrenal cortex, and by the testes in men. BioVision’s human Estradiol E2 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Estradiol E2 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Estradiol E2 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Estradiol E2 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 2 – 50 pmol/L.
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K3830-100
K3830-100? 96 wells coated with anti-mouse Estradiol antibody, 1 Microplate with 2 adhesive strips ? Mouse Estradiol standard (72 ng/L) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Serum & plasma (EDTA/Citrate), urine ? Cell culture medium, tissue homogenates and cell lysates Estradiol E2 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse Estradiol (E2 or 17β-estradiol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:2 – 50 ng/L. 100 assays. Estradiol (E2 or 17β-estradiol) is the predominant female reproductive hormone secreted by the ovaries. Smaller amounts of estradiol are also produced by the adrenal cortex and by the testes. BioVision’s mouse Estradiol E2 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Mouse Estradiol E2 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Estradiol E2 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Estradiol E2 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 2 – 50 ng/L.
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K3831-100
K3831-100? 96 wells coated with anti-rat Estradiol antibody, 1 Microplate with 2 adhesive strips ? Rat Estradiol standard (72 ng/L) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Serum & plasma (EDTA/Citrate), urine ? Cell culture medium, tissue homogenates and cell lysates Estradiol E2 (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat Estradiol (E2 or 17β-estradiol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:2 – 50 ng/L. 100 assays. Estradiol (E2 or 17β-estradiol) is the predominant female reproductive hormone secreted by the ovaries. Smaller amounts of estradiol are also produced by the adrenal cortex and by the testes. BioVision’s rat Estradiol E2 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Rat Estradiol E2 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Estradiol E2 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat Estradiol E2 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 2 – 50 ng/L.
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K4765-100
K4765-100? Fas Ligand Microplate (Item A) coated with anti-human Fas Ligand, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Fas Ligand) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody Fas Ligand (Item F), biotinylated anti-human Fas Ligand (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? UrineHuman Fas Ligand (FasL) ELISA Kit: Colorimetric Assay for Quantitative Determination of human FasL in Serum, Plasma, Urine & Cell culture supernatants. Detection Range: 2 pg/ml - 1000 pg/ml. 100 assays. BioVision’s Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand. This assay employs an antibody specific for human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of Fas Ligand is typically less than 2 pg/ml. Detection Range: 2 pg/ml - 1000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K3331-100
K3331-100? FasL Microplate coated with anti-mouse Ab against FasL, 96 wells ? Lyophilized recombinant mouse FasL standard (10 ng/vial) ? Biotinylated anti-mouse FasL antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatantsFas Ligand (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse FasL in serum, plasma & cell culture supernatants. Detection Range: 15.6-1000 pg/ml. 100 assays.BioVision’s mouse FasL (Fas Ligand, CD95L) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form rat specific for FasL coated on a 96-well plate. Standards (NSO, P132-L279) and test samples are added to the wells and FasL present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for FasL is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Fas L captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml.
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K3330-100
K3330-100? FasL Microplate coated with anti-rat Ab against FasL, 96 wells ? Lyophilized recombinant rat FasL standard (10 ng/vial) ? Biotinylated anti-rat FasL antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatantsFas Ligand (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat FasL in serum, plasma & cell culture supernatants. Detection Range: 31.2-2000 pg/ml. 100 assays.BioVision’s rat FasL ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form mouse specific for FasL coated on a 96-well plate. Standards (NSO, Q127-L278) and test samples are added to the wells and FasL present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for FasL is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat FasL captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 31.2 pg/ml – 2000 pg/ml. Sensitivity: < 10 pg/ml.
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