20-MUGHU-E01
Mumps IgG ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos. / neg. / cut off control
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20-MUMHU-E01
Mumps IgM ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos. / neg. control
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65-TBGHU-E01
Mycobacterium Tuberculosis IgG ELISA Sensitivity: Sample Types:Serum Sample Sizes:4 μL Range:
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65-TBMHU-E01
Mycobacterium Tuberculosis IgM ELISA Sensitivity: Sample Types:Serum Sample Sizes:4 μL Range:
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20-MHAHU-E01
Mycoplasma Hominis ELISA (IgA) Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:neg., cut-off, pos. control
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20-MHGHU-E01
Mycoplasma Hominis IgG ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos./neg. / cut-off control
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20-MHMHU-E01
Mycoplasma Hominis IgM ELISA Sensitivity: Sample Types:Serum Sample Sizes:10 μL Range:pos. / neg. control
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30-6630
Myeloperoxidase ELISA (MPO ELISA) Sensitivity:1.6 ng/mL Sample Types:Stool, Urine Sample Sizes:100 mg Range:1.2 - 100 ng/mL
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K3073-100
K3073-100? CD40 Microplate (Item A) coated with anti-mouse CD40, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse CD40) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. 5x concentrated ? Detection Antibody CD40 (Item F), biotinylated anti-mouse CD40 (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants CD40 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse CD40 in serum, plasma & cell culture supernatants. Detection Range: 3 pg/ml - 1500 pg/ml. 100 assays. BioVision’s mouse CD40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD40. This assay employs an antibody specific for mouse CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13,IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of CD40 is typically less than 3 pg/ml. Detection Range: 3 pg/ml – 1500 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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30-6631A
Myeloperoxidase ELISA (MPO ELISA) (Serum and Plasma) Sensitivity:1.08 ng/mL Sample Types:Plasma, Serum Sample Sizes:10 μL Range:1.9 - 30 ng/mL
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K4783-100
K4783-100? CD40L Microplate (Item A) coated with anti-human CD40L, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human CD40L) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody CD40L (Item F), biotinylated anti-human CD40L (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 600x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (EDTA & citrate) ? Cell culture supernatants ? Urine CD40 Ligand (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CD40L in serum, plasma, urine & cell culture supernatants. Detection Range: 6 pg/ml - 6000 pg/ml. 100 assays. BioVision’s Human CD40 Ligand (CD40L, CD154) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD40L. This assay employs an antibody specific for human CD40L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of CD40L is typically less than 6 pg/ml. Detection Range: 6 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K7430-100
K7430-100? Plate Coated with Cortisol Ab ? Assay Diluent ? Wash Buffer (10x) ? Standard Diluent ? Cortisol Standard (4800 ng/ml) ? Cortisol HRP Conjugate (2000x) ? TMB Substrate ? Stop Solution ? Plate Sealer ? Serum and plasma ? Cell culture supernatant Cortisol (human/mouse/rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human, mouse, rat cortisol. Sensitivity: 1 ng/ml and detection range: 5 ng/ml to 480 ng/ml. 100 assays. BioVision’s Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of Cortisol in serum and plasma. Cortisol is a steroid hormone released from the adrenal cortex in response to the hormone ACTH. It increases blood pressure and blood sugar levels, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects on numerous physiologic systems, including immune system, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. It exists in the blood as either a free form or bound to corticosteroid-binding globulin (CBG). The amount of Cortisol present in serum undergoes diurnal variation, with the highest levels present in the early morning and lower levels at night. BioVision’s Cortisol ELISA kit is a competitive enzyme immunoassay kit. The assay employs an antibody specific for cortisol coated on a 96-well plate. Cortisol in a sample or a HRP molecule which has Cortisol covalently attached to it is bound to the wells by immobilized antibody in a competitive manner. After a simultaneous incubation, the excess reagents are washed and a TMB substrate is added to the wells. Color develops in proportion to the amount of bound Cortisol HRP conjugate, and the intensity is inversely proportional to the concentration of Cortisol in the sample. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 1 ng/ml and detection range is from 5 ng/ml to 480 ng/ml. The cross-reactivity of this ELISA assay is 6.8% for prednisolone, 4.22% for cortisone, and <0.1% for other hormones, including 11-deoxycortisol, estradiol, testosterone, and progesterone. The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
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K7434-100
K7434-100? Plate Coated with Cortisol Ab ? Assay Diluent ? Wash Buffer (10x) ? Standard Diluent ? Cortisol Standard (4800 ng/ml) ? Cortisol HRP Conjugate ? TMB Substrate ? Stop Solution ? Plate SealerSalivaCortisol (Saliva) (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human salivary cortisol. Sensitivity: 1 ng/ml and detection range: 1 ng/ml to 100 ng/ml. 100 assays. BioVision’s Cortisol (Saliva) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of Cortisol in saliva. Cortisol is a steroid hormone released from the adrenal cortex in response to the hormone ACTH. It increases blood pressure and blood sugar levels, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects on numerous physiologic systems, including immune system, glucose-counter regulation, vascular tone, substrate utilization, and bone metabolism. It exists in the blood as either a free form or bound to corticosteroid-binding globulin (CBG). While only 1-10% of Cortisol in the blood exists in the unbound/free form, this fraction represents the active pool of Cortisol eliciting the biological response, and is capable of diffusing into target tissues and saliva. Studies consistently report high correlation between salivary and serum cortisol levels, indicating that salivary cortisol can reliably estimate serum Cortisol levels. BioVision’s Cortisol (Saliva) ELISA kit is a competitive enzyme immunoassay kit. The assay employs an antibody specific for cortisol coated on a 96-well plate. Cortisol in a sample and HRP labeled Cortisol compete for binding to the immobilized antibody. After simultaneous incubation, the excess reagents are washed away and a TMB substrate is added to the wells. Color develops in proportion to the amount of bound Cortisol HRP conjugate, and the intensity is inversely proportional to the concentration of Cortisol in the sample. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 1 ng/ml and detection range is from 1 ng/ml to 100 ng/ml. The cross-reactivity of this ELISA assay is 6.8% for prednisolone, 4.22% for Cortisone, and <0.1% for other hormones, including 11-deoxycortisol, estradiol, testosterone, and progesterone.
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K4757-100
K4757-100? Serum & plasma ? Cell culture media etc. C-Peptide (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of C-Peptide in Serum, Plasma, Cell culture supernatants etc. Detection range: 0.1-1000 ng/ml. 100 assays.BioVision’s C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- C-Peptide antibody, both biotinylated C-Peptide peptide and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide peptide can be established and the concentration of C-Peptide peptide in the samples can be calculated accordingly. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain. The minimum detectable concentration of C-Peptide is 772 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4387-100
K4387-100? Microplate coated with anti-human SDF-1 alpha ? Wash Buffer (20x) (Item B) ? Human SDF-1 alpha Standard (Item C) ? Assay Diluent A (Item D) (0.09% NaN3) ? Assay Diluent B (Item E) (5x concentrated) ? Detection Antibody SDF-1 alpha (Item F) ? HRP-Streptavidin (Item G), (200x) ? TMB (Item H) ? Stop Solution (Item I) ? Plasma (human SDF-1 alpha conc. is pretty low in normal plasma, it may not be detected. serum samples are not recommended) ? Cell culture supernatants ? Urine CXCL12/SDF-1 alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CXCL12/SDF-1 alpha in plasma, cell culture supernatants & urine. Detection Range: 80 pg/ml – 6,000 pg/ml. 100 assays.BioVision’s Human SDF-1 (Stromal cell-derived factor-1) alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SDF-1 alpha. This assay employs an antibody specific for human SDF-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SDF-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. Sensitivity: 80 pg/ml. Detection Range: 80 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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