K1051-100
K1051-100? TNF-α mAb coated plate, 96 wells ? Mouse TNF-α standard (10 ng/vial) ? Biotinylated anti-mouse TNF-α Ab ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB (Colorless) ? TMB stop solution ? Serum & plasma (heparin, EDTA) ? Cell culture supernatants TNF alpha (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse TNF alpha in serum, plasma & cell culture supernatants.Sensitivity: < 1 pg/ml. 100 assays. BioVision’s mouse TNF alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for mouse TNF alpha coated on a 96-well plate. Standards and test samples are added to the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse TNF alpha captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (mouse serum, plasma). Sensitivity: < 1 pg/ml.
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K1052-100
K1052-100? TNF alpha Microplate coated with anti-rat monoclonal Ab against TNF alpha, 96 wells ? Lyophilized recombinant rat TNF alpha standard (10 ng/vial) ? Biotinylated anti-rat TNF alpha antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA) ? Cell culture supernatants TNF alpha (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat TNF alpha in serum, plasma & cell culture supernatants.Sensitivity: < 1 pg/ml. 100 assays. BioVision’s rat TNF alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for rat TNF alpha coated on a 96-well plate. Standards and test samples are added to the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat TNF alpha captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (rat serum, plasma). Sensitivity: < 1 pg/ml.
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K4779-100
K4779-100? TNF-alpha Microplate (Item A) coated with anti-human TNF-alpha, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human TNF-alpha) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody TNF-alpha (Item F), biotinylated anti-human TNF-alpha (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 600x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human TNF-alpha concentration is pretty low in normal serum & plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine TNF-alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TNF-alpha in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 30 pg/ml - 6000 pg/ml. 100 assays. BioVision’s Human TNF-alpha (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-alpha. This assay employs an antibody specific for human TNF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ1, TGFβ2, TGFβ3, VEGF. The minimum detectable dose of TNF-alpha is typically less than 30 pg/ml. Detection Range: 30 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4780-100
K4780-100? TNF-beta Microplate (Item A) coated with anti-human TNF-beta, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human TNF-beta) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody TNF-beta (Item F), biotinylated anti-human TNF-beta (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 700x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human TNF-beta concentration is pretty low in normal serum & plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine TNF-beta (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TNF-beta in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 60 pg/ml - 18000 pg/ml. 100 assays. BioVision’s Human TNF-beta (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-beta. This assay employs an antibody specific for human TNF-beta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL- 12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TPO, VEGF. The minimum detectable dose of TNF-beta is typically less than 60 pg/ml. Detection Range: 60 pg/ml – 18,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4753-100
K4753-100? Serum & plasma ? Cell culture supernatants TPO (Mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse TPO in Serum, Plasma & Cell culture supernatants. Detection Limit: less than 30 pg/ml. 100 assays.BioVision’s Mouse TPO ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TPO. This assay employs an antibody specific for mouse TPO coated on a 96-well plate. Standards and samples are pipetted into the wells and TPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TPO is typically less than 30 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K7423-100
K7423-100? Plate coated with T3 Ab ? T3 Standard ? Conjugate Buffer ? T3 Enzyme Conjugate Conc. ? Wash Concentrate (20X) ? TMB Substrate ? Stop SolutionSerum or plasma Triiodothyronine (T3) (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Triiodothyronine in serum or plasma. Sensitivity: 0.04 ng/ml. 100 assays. Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients, graves disease, and pregnancy. BioVision’s human Triiodothyronine (T3) kit is a solid phase competitive ELISA Kit. The samples and T3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. T3 in the sample competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
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K7422-100
K7422-100? Plate coated with T3 Ab ? T3 Standard ? Assay Diluent ? T3 Enzyme Conjugate Conc. ? Wash Concentrate (20X) ? TMB Substrate ? Stop SolutionSerum or plasma Triiodothyronine (T3) (Mouse/Rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of mouse/rat Triiodothyronine in serum or plasma. Sensitivity: 0.186 ng/ml. 100 assays. Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s mouse/rat Triiodothyronine (T3) kit is a solid phase competitive ELISA Kit. The samples, and T3 enzyme conjugate are added to the wells coated with anti-T3 polyclonal antibody. T3 in the sample competes with a T3 enzyme (HRP) conjugate for binding sites. Unbound T3 and T3 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
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K4917-100
K4917-100? 1 plate coated with human Vaspin Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Conjugated anti-rabbit IgG) ? 1 vial human Vaspin Standard (lyophilized) ? 1 vial human Vaspin QC sample (lyophilized) ? 1 bottle TMB Substrate Solution ? 1 bottle Stop Solution ? 3 plate sealers (plastic film)Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsVaspin (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Vaspin in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.016 – 1 ng/ml and Detection limit 12 pg/ml. 100 Assays. Vaspin, designated as visceral adipose tissue-derived serpin, is a serpin whose expression is restricted to visceral adipose tissue. Vaspin seems to retain the serpin signature conformation fold consisting of three β-sheets and nine α-helices as well as a reactive loop site that interacts with its cognate serine protease unidentified hitherto. Vaspin is remarkably upregulated at high-fat high-sucrose (HFHS) diet and at multiple metabolic dysfunctions like obesity or insulin resistance. Administration of thiazolidinedione (TZD) caused a significant induction of serum vaspin in mice. These facts imply that upregulation of vaspin may be a compensatory response to antagonize the action of other unknown proteases upregulated in obesity or insulin resistance. Indeed, administration of recombinant vaspin to obese mice fed with HFHS chow improved glucose tolerance and insulin sensitivity. Vaspin seems to directly act on white adipose tissue. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human vaspin in biological fluids. A monoclonal antibody specific for vaspin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, vaspin is recognized by the addition of a purified polyclonal antibody specific for vaspin (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of vaspin in the samples. The assay range is 0.016 – 1 ng/ml Vaspin/ml. The lowest level of vaspin that can be detected by this assay is 12 pg/ml.
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K7211-100
K7211-100? VCAM-1 Microplate coated with anti-human monoclonal Ab against VCAM-1, 96 wells ? Lyophilized recombinant human VCAM-1 standard (10 ng/vial) ? Biotinylated anti- human VCAM-1antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatantsVCAM-1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human VCAM-1 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s human VCAM-1 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for human VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human VCAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.
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K7212-100
K7212-100? VCAM-1 Microplate coated with anti-mouse monoclonal Ab against VCAM-1, 96 wells ? Lyophilized recombinant mouse VCAM-1 standard (10 ng/vial) ? Biotinylated anti- mouse VCAM-1antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatantsVCAM-1 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse VCAM-1 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s mouse vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for mouse VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VCAM-1 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 5 pg/ml.
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K5363-100
K5363-100? Plate coated with anti-human VEGF antibody, 96-wells ? Human VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-human VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatants ? UrineVEGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 31.2 pg/ml – 2000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. VEGF mediates progression of Diabetic retinopathy as well as cancer metastasis. BioVision’s human VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 31.2 pg/ml – 2000 pg/ml. Sensitivity: < 1 pg/ml.
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K5364-100
K5364-100? Plate coated with anti-mouse VEGF antibody, 96-wells ? Mouse VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-mouse VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma ? Cell culture supernatants ? UrineVEGF (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of murine VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 15.6 pg/ml – 1000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s mouse VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VEGF captured onto the plate. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 2 pg/ml.
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K5365-100
K5365-100? Plate coated with anti-rat VEGF antibody, 96-wells ? Rat VEGF standard (10 ng/vial), lyophilized ? Biotinylated anti-rat VEGF antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma ? Cell culture supernatants ? UrineVEGF (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 15.6 pg/ml – 1000 pg/ml. 100 assays. Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s rat VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat VEGF captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
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K4806-100
K4806-100? 96 wells coated with anti-human Vit. D3 antibody, 1 Microplate ? Human Vit. D3 standard (0.9 ng/μl) ? HRP-conjugate reagent ? Standard Diluent ? Sample diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Sealed Bag ? Plate cover ? Serum & plasma (EDTA/Citrate) ? Urine ? Cell culture medium, Tissue homogenates and cell lysatesVitamin D3 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Vitamin D3 (cholecalciferol) in serum, plasma, urine, cell culture media and cell/tissue lysates. Detection Range:200 – 800 ug/L. 100 assays. Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. BioVision’s human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 – 800 μg/L.
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K3381-100
K3381-100? 96 wells coated with anti-human β-catenin antibody, 1 Microplate ? Human β-catenin standard (18 ng/ml) ? HRP-conjugate reagent ? Standard Diluent ? Sample Diluent ? Chromogen Solution A ? Chromogen Solution B ? Stop Solution ? Wash Solution (30x stock) ? Sealed Bag ? Plate cover ? Serum and other biological fluids ? Cell culture medium, tissue homogenates and cell lysates β-catenin (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human β-catenin in serum, biological fluids, cell culture media and cell/tissue lysates. Detection Range: 0.156 – 10 ng/ml. 100 assays. β-catenin (cadherin-associated protein beta, 88 kDa) is a dual function protein, regulating the coordination of cell–cell adhesion and gene transcription via the Wnt signaling pathway. Mutations and overexpression of β-catenin are associated with many cancers, including hepatocellular, colorectal, lung, breast, ovarian and endometrial cancers. BioVision’s human β-catenin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human β-catenin antibody is coated on a 96-well plate. Standards and test samples are added to the wells and β-catenin present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human β-catenin captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 0.156 – 10 ng/ml.
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