5004-100
BDNF Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot Western blot analysis (0.5-4 μg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. Recombinant human BDNF (Cat.# 4004-10) can be used as a positive control.
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5005-100
Jacalin Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:All Application:Western Blot Western Blot analysis (1-4 μg/ml). However, the optimal concentrations should be determined individually.
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BE0099-5MG
InVivoMab recombinant CTLA-4-Ig (hum./hum.) bioxcell InVivoMab-北京博迈斯科技发展有限公司
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K4790-100
K4790-100? Ghrelin Microplate (Item A) coated with secondary antibody, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Lyophilized Standard Ghrelin Peptide (Item C) ? Lyophilized anti-Ghrelin Polyclonal antibody (Item N) ? 1 X Assay Diluent E (Item R) for Standard/Sample (Serum or plasma, cell culture medium etc.), 25 ml/vial ? Lyophilized Biotinylated Ghrelin peptide (Item F) ? HRP-Streptavidin Concentrate (Item G), 100x concentrated ? Lyophilized Positive Control (Item M) ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture media or other sample types Ghrelin (human, mouse, rat) EIA Kit: Colorimetric Assay for Quantitative measurement of human, mouse, rat ghrelin in serum, plasma, cell culture supernatants & other samples. Detection Range: 0.1-1,000 ng/ml. 100 assays. BioVision’s Ghrelin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Ghrelin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Ghrelin antibody, both biotinylated Ghrelin peptide and peptide standard or targeted peptide in samples interacts competitively with the Ghrelin antibody. Uncompeted (bound) biotinylated Ghrelin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Ghrelin peptide in the standard or samples. This is due to the competitive binding of Ghrelin antibody between biotinylated Ghrelin peptide and peptides in standard or samples. A standard curve of known concentration of Ghrelin peptide can be established and the concentration of Ghrelin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Ghrelin is 161 pg/ml or 12.46 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%. This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Nesfatin, Angiotensin II, NPY and APC.
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K4756-100
K4756-100? Serum & plasma ? Cell culture media etc. Glucagon (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of Glucagon in Serum, Plasma, Cell culture supernatants etc.. Detection range: 1-1000 pg/ml. 100 assays.BioVision’s Glucagon Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Glucagon antibody, both biotinylated Glucagon peptide and peptide standard or targeted peptide in samples interacts competitively with the Glucagon antibody. Uncompeted (bound) biotinylated Glucagon peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Glucagon peptide in the standard or samples. This is due to the competitive binding to Glucagon antibody between biotinylated Glucagon peptide and peptides in standard or samples. A standard curve of known concentration of Glucagon peptide can be established and the concentration of Glucagon peptide in the samples can be calculated accordingly. The minimum detectable concentration of Glucagon is 0.97 pg/ml. The detection range for the kit is 1-1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4781-100
K4781-100? HGF Microplate (Item A) coated with anti-human HGF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human HGF) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody HGF (Item F), biotinylated anti-human HGF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (EDTA & citrate) ? Cell culture supernatants ? Urine HGF (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human HGF in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 3 pg/ml - 2000 pg/ml. 100 assays. BioVision’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF). The minimum detectable dose of HGF is typically less than 3 pg/ml. Detection Range: 3 pg/ml – 2,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4782-100
K4782-100? HGF Microplate (Item A) coated with anti-human HGF, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human HGF) ? Sample Diluent Buffer (Item D) for Standard/Sample (cell lysate/tissue lysate) diluent (5x concentrated) ? Assay Diluent (Item E) for Detection Antibody & HRP-Streptavidin concentrate diluent (5x concentrated) ? Detection Antibody HGF (Item F), biotinylated anti-human HGF (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Cell Lysate buffer (Item J) (2x concentrated) ? Cell lysates ? Tissue lysates HGF (human) ELISA Kit (For Lysates): Colorimetric Assay for Quantitative measurement of human HGF in Cell & tissue lysates. Detection Limit: less than 3 pg/ml. 100 assays. BioVision’s Human HGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HGF. This assay employs an antibody specific for human HGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of HGF is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF).
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K4117-100
K4117-100? Plate coated with anti-mouse IFN-gamma ? Wash Buffer (20x) (Item B) ? Mouse IFN-gamma Standard (Item C) ? Assay Diluent A (Item D) (0.09% NaN3) ? Assay Diluent B (Item E) (5x) ? Detection Antibody IFN-gamma (Item F) ? HRP-Streptavidin (Item G) (400x) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants IFN-gamma (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse IFN-gamma in Serum, Plasma, & Cell culture supernatants. Detection Range: 5 pg/ml – 2000 pg/ml. 100 assays. IFN-γ is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. It plays an important role in the immune IFN-γresponse. IFN-γ is a modulator of T-cell growth and functional differentiation. It is a growth-promoting factor for T-lymphocytes and potentiates the response of these cells to mitogens or growth factors. BioVision’s mouse IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IFN-gamma. This assay employs an antibody specific for mouse IFN- gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN- gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IFN- gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN- gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. Sensitivity: 5 pg/ml. Detection Range: 5 pg/ml – 2000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4773-100
K4773-100? IFN-γ Microplate (Item A) coated with anti-human IFN-γ, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IFN-γ) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody IFN-γ (Item F), biotinylated anti-human IFN-γ (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma (human IFN-γ concentration is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine IFN-gamma (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IFN-gamma in Serum, Plasma, urine & Cell culture supernatants. Detection Range: 15 pg/ml - 15000 pg/ml. 100 assays. BioVision’s Human IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-γ. This assay employs an antibody specific for human IFN-γ coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-γ present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN-γ antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of IFN-γ is typically less than 15 pg/ml. Detection Range: 15 pg/ml - 15000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4774-100
K4774-100? IFN-gamma Microplate (Item A) coated with anti-human IFN-gamma, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IFN-gamma) ? Sample Diluent Buffer (Item D) for Standard/Sample (cell lysate/tissue lysate) diluent (5x concentrated) ? Assay Diluent (Item E) for Detection Antibody & HRP-Streptavidin concentrate diluent (5x concentrated) ? Detection Antibody IFN-γ (Item F), biotinylated anti-human IFN-gamma (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 400x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Cell Lysate buffer (Item J) (2x concentrated) ? Cell lysates ? Tissue lysates IFN-gamma (human) ELISA Kit (For Lysates): Colorimetric Assay for Quantitative measurement of human IFN-gamma in cell & tissue lysates. Detection limit: 15 pg/ml. 100 assays. BioVision’s Human IFN-gamma ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-gamma. This assay employs an antibody specific for human IFN-gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti- human IFN-gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IFN-gamma is typically less than 15 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, MCP1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
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K4775-100
K4775-100? IGF-I Ab-coated Microplate (Item A), 96 wells ? Wash Buffer (20x) (Item B) ? Human IGF-I Standard (Item C) ? Assay Diluent C (Item L) ? Detection Antibody (Item F) ? HRP-Streptavidin (120x) (Item G) ? TMB (Item H) ? Stop Solution (Item I) ? Serum & plasma ? Cell culture supernatants ? Urine IGF-1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IGF-1 in serum, plasma, urine & cell culture supernatants. Detection Range: 0.1 ng/ml - 30 ng/ml. 100 assays. BioVision’s human IGF-I ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I. This assay employs an antibody specific for human IGF-I coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGF-I antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit only can detect a free form of human IGF-I & shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of IGF-I is typically less than 0.1 ng/ml. Detection Range: 0.1 ng/ml - 30 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4776-100
K4776-100? IGF-I sR Microplate (Item A) coated with anti-human IGF-I sR, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human IGF-I sR) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody IGF-I sR (Item F), biotinylated anti-human IGF-I sR (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 300x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine IGF-1 sR (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human IGF-1 sR in serum, plasma, urine & cell culture supernatants. Detection Range: 6 pg/ml - 6000 pg/ml. 100 assays. BioVision’s human IGF-I sR ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGF-I sR. This assay employs an antibody specific for human IGF-I sR coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF-I sR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGF-I sR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF-I sR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. Sensitivity: 6 pg/ml. Detection Range: 6 pg/ml - 6000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4739-100
K4739-100? IL-15 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human IL-15. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials of recombinant human IL-15. ? Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody IL-15 (Item F): 2 vial of biotinylated anti-human IL-15 (each vial is enough to assay half microplate). ? HRP-Streptavidin concentrate (Item G): 200 μl of 200x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’- tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum, plasma (collect plasma using EDTA or heparin as an anticoagulant. Citrate plasma is not recommended for this assay) ? Cell culture supernatants ? Urine IL-15 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of Human IL-15 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 3 pg/ml. 100 assays.IL-15 is a recently identified novel cytokine that shares many biological properties with IL-2, including T-Cell, B-Cell and NKcell-stimulatory activities. BioVision’s Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-15. This assay employs an antibody specific for human IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-15 is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 β, MIP-1, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-alpha, TNF-β, TPO, VEGF).
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K4740-100
K4740-100? IL-17 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human IL-17. ? Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. ? Standards (Item C): 2 vials, recombinant human IL-17. ? Assay Diluent A (Item D): 30 ml of animal serum with 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. ? Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. ? Detection Antibody IL-17 (Item F): 2 vial of biotinylated anti-human IL-17 (each vial is enough to assay half microplate). ? HRP-Streptavidin Concentrate (Item G): 200 μl of 120x concentrated HRP-conjugated streptavidin. ? TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. ? Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. ? Serum & plasma (human IL-17 concentration is pretty low in normal serum/plasma, it may not be detected in this assay) ? Cell culture supernatants ? Urine IL-17 (human) ELISA Kit: Colorimetric Assay for Quantitative Determination of Human IL-17 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: less than 80 pg/ml. 100 assays.BioVision’s Human IL-17 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-17. This assay employs an antibody specific for human IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-17 is typically less than 80 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1?, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1β, MIP-1, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-alpha, TNF-β, TPO, VEGF).
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