5253-100
MIP-2 Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Mouse, rat Application:Western blot, Neutralization, ELISA Western blot analysis (0.5-4 μg/ml), immunoprecipitation (4-8 μg/ml), and neutralization. However, the optimal conditions should be determined individually.
More
5286-100
CITED2 Antibody (NT) Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human, mouse, rat Application:Western blot, ELISA Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
More
5287-100
CRIM1 Antibody (CT) Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot, ELISA Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
More
5300-100
KLF4 mAntibody (4G6E11) Host:Mouse Antibody Type:Monoclonal Species Reactivity:Human, Mouse, Rat Application:Western blot, ELISA Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
More
5302-100
LMX1B Antibody (CT) Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human, Mouse, Rat Application:Western blot, ELISA Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
More
5306-200
NT-3 Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot The antibody can be used in Western Blot analysis (0.5-4 μg/ml). However, the optimal conditions should be determined individually. Recombinant human NT-3 (Cat.# 4306-10) can be used as a positive control.
More
5307-100
NGN2 Antibody (CT) Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human, Mouse, Rat Application:Western blot, ELISA Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
More
K7431-100
K7431-100? Microwells coated with Streptavidin ? PSA Standard* ? Anti-PSA Conjugate Reagent ? Wash Buffer (20X) ? TMB Substrate ? Stop Solution Serum Prostate Specific Antigen (Total, human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human total Prostate Specific Antigen. Sensitivity: 0.0144 ng/ml. 100 assays. Prostate Specific Antigen (PSA) is useful in the management of patients with prostate cancer. It is a single chain glycoprotein produced by epithelial cells of the prostate gland. The measurement of serum PSA has become the most accepted test to indicate men who are at risk of having prostate cancer and who should be examined by other tests. Using a cut-off of 4 ng/ml, 92% of men over 50 years of age with malignant prostatic tissues, 8% of healthy men and 28% of men with benign prostate hyperplasia (BPH) test positive for PSA. Three major forms of PSA exist in the serum: free PSA, bound PSA and complex PSA. Bound PSA is found in higher concentrations in patients with prostate cancer; whereas, free PSA is detected in higher concentrations in patients with BPH. If the free PSA to total PSA ratio is >25%, it is unlikely that the patient has prostate cancer; whereas, if free PSA is <16% then prostate cancer is likely to be the cause. Serial measurement of PSA concentration in the serum is an important tool in monitoring patients with prostatic cancer and determining the potential and actual effectiveness of surgery or other therapies, or may allow for earlier discovery of residual or recurrent carcinoma after radical prostatectomy or radiotherapy. BioVision’s PSA ELISA kit is a solid phase assay based on a streptavidin-biotin principle. The standards, samples and a reagent mixture of Anti-PSA Enzyme and Biotin conjugates (conjugate reagent) are added into the wells, coated with Streptavidin. PSA in the serum sample forms a sandwich between two highly specific Anti-PSA antibodies, labeled with Biotin and HRP. Simultaneously, the biotinylated antibody is immobilized onto the well through a high affinity Streptavidin-Biotin interaction. Unbound protein and excess biotin/enzyme conjugated reagent are washed off, by washing buffer. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of PSA in the samples. A standard curve is prepared relating color intensity to the concentration of the PSA.
More
K4771-100
K4771-100? RANTES Microplate (Item A) coated with anti-mouse RANTES, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant mouse RANTES) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent C (Item L) for Standard/Sample (cell culture medium) diluent ? Assay Diluent B (Item E) for Detection Antibody & HRP-Streptavidin diluent. (5x concentrated) ? Detection Antibody RANTES (Item F), biotinylated anti-mouse RANTES (each vial enough for half plate) ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants RANTES (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse RANTES in Serum, Plasma & Cell culture supernatants. Detection Range: 1 pg/ml - 200 pg/ml. 100 assays. BioVision’s Mouse RANTES (Regulated upon activation, Normal T cell Expressed and presumably Secreted) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RANTES. This assay employs an antibody specific for mouse RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF-α, TNF RI, TNF RII, TPO, VCAM-1, VEGF. The minimum detectable dose of RANTES is typically less than 1 pg/ml. Detection Range: 1 pg/ml - 200 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
More
K4911-100
K4911-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Human RBP4 Standard (lyophilized, 5 μg) ? Human RBP4 QC Sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? Plate Sealers Serum, urine, or cell culture supernatantRBP4 (human) Competitive ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of human RBP4 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.001 – 5 μg RBP4/ml and detection limit 1 ng/ml. 100 Assays.Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, the expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (human) ELISA Kit is to be used for the in vitro quantitative determination of human RBP4 in serum, urine and cell culture supernatant. This assay is a competitive ELISA which utilizies a 96-well microtiter plate which was pre-coated with a human RBP4. A purified polyclonal which recognizing native human RBP4 reacts with a series of predetermined recombinant human RBP4 standard proteins or the test samples under competition in the human RBP4-coated plate. Their relative reactivity is plotted with that of the standard proteins. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, rat adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-23, human IL-33, human GPX3, human Nampt, human ANG1, human ANG2, human ANGPTL3, human ANGPTL4, human ANGPTL6, human FABP4, human RELM-β, rat RELM-α, mouse Nampt, human PAI-1. The assay range is 0.001 – 5 μg RBP4/ml and a detection limit of 1 ng/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
More
K4912-100
K4912-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Human RBP4 Standard (lyophilized, 50 ng) ? Human RBP4 QC Sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? Plate Sealers Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsRBP4 (human) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human RBP4 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.39 – 25 ng RBP4/ml and Detection limit 380 pg/ml. 100 Assays. Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (human) ELISA Kit is to be used for the in vitro quantitative determination of human RBP4 in biological fluids. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3’,5,5’-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-33, human GPX3, human progranulin, human FABP4, human ANGPTL3, human ANGPTL4, human ANG1, human ANG2, human Nampt, mouse Nampt. The assay range is 0.39 – 25 ng RBP4/ml and a detection limit of 380 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
More
K4913-100
K4913-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Mouse RBP4 Standard (lyophilized, 24 ng) ? Mouse RBP4 QC Sample (lyophilized) ? Substrate Solution I (TMB) ? Substrate Solution II (Peroxidase) ? Stop Solution ? Plate Sealers Serum, urine, or cell culture supernatantRBP4 (mouse/rat) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Mouse or Rat RBP4 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.188 – 12 ng RBP4/ml and Detection limit 60 pg/ml. 100 Assays.Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (mouse/rat) ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3’,5,5’-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not cross-react with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-α, mouse RELM-β, human leptin, rat RELM-α, mouse 41BBL, mouse CD137, mouse Tim3, human TNF-α. The assay range is 0.188 – 12 ng RBP4/ml and a detection limit of 60 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
More
K4919-100
K4919-100? 1 plate coated with human Resistin Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial human Resistin Standard (lyophilized) ? 1 vial human Resistin QC sample (lyophilized) ? 1 bottle Substrate Solution I (TMB) ? 1 bottle Substrate Solution II (Peroxidase) ? 1 bottle Stop SolutionCell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsResistin (human) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Resistin in Serum, Plasma or Cell Culture Supernatant. Assay Range 0.125–8 ng/ml and Detection limit 100 pg/ml.100 Assays. Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.125–8 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
More
K4767-100
K4767-100? Resistin Microplate (Item A) coated with secondary antibody, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standard Resistin Peptide (Item C) (10 μl/vial) ? Anti-Resistin polyclonal antibody (item N) (5 μl/vial) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium or other sample) diluent. (5x concentrated) ? Biotinylated Resistin peptide (Item F), (20 μl/vial) ? HRP-Streptavidin Concentrate (Item G), 160x concentrated ? Positive Control (Item M) (100 μl) ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Culture supernatants & cell lysates Resistin (human/mouse/rat) ELIA Kit: Colorimetric Assay for Quantitative Determination of human/mouse/rat Resistin in Serum, Plasma, Cell lysates & Cell culture supernatants. Detection Range: 0.1-1,000 ng/ml. 100 assays. BioVision’s Resistin [XCP-1 (CEBPE regulated myeloid-specific secreted cysteine-rich protein precursor 1), FIZZ3 (found in inflammatory zone 3), or ADSF (adipocyte-specific secretory factor)] Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Resistin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti-Resistin antibody, both biotinylated Resistin peptide and peptide standard or targeted peptide in samples interacts competitively with the Resistin antibody. Uncompeted (bound) biotinylated Resistin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Resistin peptide in the standard or samples. This is due to the competitive binding to Resistin antibody between biotinylated Resistin peptide and peptides in standard or samples. A standard curve of known concentration of Resistin peptide can be established and the concentration of Resistin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Resistin is 446 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%. This EIA kit detects Resistin (90 aa). No other active isoforms have been reported. This kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
More
K4920-100
K4920-100? 1 plate coated with mouse Resistin Antibody ? 1 bottle Wash Buffer 10X ? 1 bottle Diluent 5X ? 1 bottle Detection Antibody ? 1 vial Detector 100X (HRP Labeled Streptavidin) ? 1 vial mouse Resistin Standard (lyophilized) ? 1 vial mouse Resistin QC sample (lyophilized) ? 1 bottle Substrate Solution I (TMB) ? 1 bottle Substrate Solution II (Peroxidase) ? 1 bottle Stop SolutionCell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluidsResistin (mouse) Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Mouse Resistin in Serum, Plasma or Cell Culture Supernatant. Assay Range 0.47 – 30 ng/ml and Detection limit 100 pg/ml. 100 Assays. Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.47 – 30 ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100 pg/ml.
More
|
|