K4923-100
K4923-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Lysis Buffer (10X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Human Sirtuin 1 Standard (lyophilized, 4 ng) ? Human Sirtuin 1 QC Sample (lyophilized) ? TMB Substrate Solution ? Stop Solution ? Plate SealersCell lysatesSirtuin 1 (human intracellular) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Sirtuin 1 in cells. Assay Range 0.031 – 2 ng/ml and Detection limit 30 pg/ml.100 Assays. Sirtuin 1 is the human ortholog for the yeast Sir2 (silent information regulator 2) protein regulating epigenetic gene silencing as a possible antiaging effect. Sirtuin 1 is an NAD(+)-dependent histone deacetylase, which deacetylate lysines 9 and 14 of histone H3 and lysine-16 of histone H4, involved in various cellular functions such as transcription, energy sensing, and differentiation. Sirtuin1 plays an important role in regulating adipogenesis via repression of PPAR and the gluconeogenic/glycolytic pathways in liver in response to fasting signals through the transcriptional coactivator PGC1A deacetylated at specific lysine residues in an NAD(+)-dependent manner. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human Sirtuin 1 in cells. A monoclonal antibody specific for Sirtuin 1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 1 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of Sirtuin 1 in the samples. This ELISA is specific for the measurement of natural and recombinant human Sirtuin 1. It does not cross-react with human Sirtuin 2, human Sirtuin 5, human Sirtuin 6, human adiponectin, human resistin, human RBP4, human vaspin, human progranulin, human GPX3, human FTO, human Nampt, human leptin, mouse FTO, mouse Nampt. The assay range is 0.031 – 2 ng Sirtuin 1/ml and a detection limit of 30 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
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K4924-100
K4924-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Lysis Buffer (10X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Human Sirtuin 2 Standard (lyophilized, 16 ng) ? Human Sirtuin 2 QC Sample (lyophilized) ? TMB Substrate Solution ? Stop Solution ? Plate SealersCell lysatesSirtuin 2 (human intracellular) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Sirtuin 2 in cells. Assay Range 0.125 – 8 ng/ml and Detection limit 80 pg/ml.100 Assays. Sirtuin 2 is a NAD-dependent histone deacetylase (39.5 kD) largely localized at the cytoplasm where it is physically associated with microtubules. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo and colocalized and interacted in vivo with HDAC6, another tubulin deacetylase. It has been also shown that Sirtuin 2 is a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human Sirtuin 2 in cells. A monoclonal antibody specific for Sirtuin 2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 2 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 2 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of Sirtuin 2 in the samples. This ELISA is specific for the measurement of natural and recombinant human Sirtuin 2. It does not cross-react with human Sirtuin 1, human Sirtuin 5, human Sirtuin 6, human adiponectin, human resistin, human RBP4, human vaspin, human progranulin, human GPX3, human FTO, human Nampt, human leptin, mouse FTO, mouse Nampt. The assay range is 0.125 – 8 ng Sirtuin 2/ml and a detection limit of 80 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
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K7417-100
K7417-100? Microplate coated with Mouse Anti-Testosterone, 96 wells ? Standard: (0.5 ml) (ready to use) ? Assay Diluent ? Enzyme Conjugate (20X) ? Wash Buffer (20X) ? TMB Substrate (ready to use) ? Stop Solution (ready to use) Serum or plasma Testosterone (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human Testosterone in serum or plasma. 100 assays. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, or chidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. BioVision’s Testosterone EIA kit is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of mouse anti-Testosterone. In the incubation, mouse anti-Testosterone coated wells are incubated with 25 μl of Testosterone standards, controls, samples & 100 μl Testosterone-HRP conjugate reagent at room temperature for 60 min. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 min., resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
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K7418-100
K7418-100? Microplate coated with Goat Anti-Rabbit IgG, 96 wells ? Standard: (0.5 ml) (ready to use) ? Rabbit Anti-Testosterone Reagent ? Assay Diluent ? Enzyme Conjugate (20X) ? Wash Buffer (20X) ? TMB Substrate (ready to use) ? Stop Solution (ready to use) Serum or plasma Testosterone (mouse/rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of mouse/rat Testosterone in serum or plasma. 100 assays. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, or chidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. BioVision’s Testosterone EIA kit is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25 μl of Testosterone standards, controls, samples, 100 μl Testosterone-HRP conjugate reagent and 50 μl rabbit anti-testosterone reagent at room temperature for 60 min. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 min., resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
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K4339-100
K4339-100? TGF-alpha Microplate coated with anti-human Ab against TGF-alpha, 96 wells ? Lyophilized recombinant human TGF-alpha standard (10 ng/vial) ? Biotinylated anti-human TGF-alpha antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution? Serum & plasma (heparin, EDTA) ? Cell culture supernatants ? Human milk TGF-alpha (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TGF-alpha in serum, plasma, cell culture supernatants & human milk. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.BioVision’s human TGF-alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from mouse specific for TGF-alpha coated on a 96-well plate. Standards and test samples are added to the wells and TGF-alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TGF-alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-alpha captured onto the plate. This ELISA kit shows cross-reactivity with TGFβ2, TGFβ3 & TGFβ5 <1%. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
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K4342-100
K4342-100? 96 wells coated with anti-human TGF-β1 antibody, 1 Microplate ? Lyophilized recombinant human TGF-β1 (25KDa) standard (10 ng/vial) ? Biotinylated anti-human TGF-β1 antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (EDTA) ? Cell culture supernatants, urine TGF-beta 1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TGF-beta 1 in serum, plasma, cell culture supernatants & human urine. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.TGF-β1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. BioVision’s human TGF-β1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human TGF-β1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-β1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-β1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-β1 captured onto the plate. This ELISA kit shows <1% cross-reactivity with TGF-β2, TGF-β3 and TGF-β5. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
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K4343-100
K4343-100? 96 wells coated with anti-mouse TGF-β1 antibody, 1 Microplate ? Lyophilized recombinant mouse TGF-β1 (25KDa) standard (10 ng/vial) ? Biotinylated anti-mouse TGF-β1 antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (EDTA) ? Cell culture supernatants, urine TGF-beta 1 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse TGF-beta 1 in serum, plasma, cell culture supernatants & urine. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.TGF-β1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. BioVision’s mouse TGF-β1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse TGF-β1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-β1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-β1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse TGF-β1 captured onto the plate. This ELISA kit shows <1% cross-reactivity with TGF-β2, TGF-β3 and TGF-β5. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 2 pg/ml.
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K4344-100
K4344-100? 96 wells coated with anti-rat TGF-β1 antibody, 1 Microplate ? Lyophilized recombinant rat TGF-β1 (25KDa) standard (10 ng/vial) ? Biotinylated anti-rat TGF-β1 antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (EDTA) ? Cell culture supernatants, urine TGF-beta 1 (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat TGF-beta 1 in serum, plasma, cell culture supernatants & urine. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.TGF-β1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. BioVision’s rat TGF-β1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat TGF-β1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-β1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-β1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat TGF-β1 captured onto the plate. This ELISA kit shows <1% cross-reactivity with TGF-β2, TGF-β3 and TGF-β5. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
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K7413-100
K7413-100? Microplate coated with T4 MAb, 96 wells ? T4 Standard: (0.5 ml) (ready to use) ? Conjugate Buffer ? T4 Enzyme (HRP) Conjugate Concentrate ? Wash Concentrate (20X) ? TMB Substrate (ready to use) ? Stop Solution (ready to use) ? Serum or plasma Thyroxine (T4) (human) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of human thyroxine in serum or plasma. 100 assays. T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s human thyroxine (T4) kit is a solid phase competitive ELISA Kit. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.
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K7421-100
K7421-100? Plate coated with T4 Ab ? T4 Standard ? Assay Diluent ? T4 Enzyme Conjugate Conc. ? Wash Concentrate (20X) ? TMB Substrate ? Stop SolutionSerum or plasma Thyroxine (T4) (Mouse/Rat) ELISA Kit: Rapid & convenient colorimetric assay for quantitative measurement of mouse/rat thyroxine in serum or plasma. Sensitivity: 0.467 μg/dl. 100 assays. T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s mouse/rat thyroxine (T4) kit is a solid phase competitive ELISA Kit. The samples, and T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.
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K4750-100
K4750-100? Serum & plasma ? Cell culture supernatants TIMP-1 (Mouse) ELISA Kit: Colorimetric Assay for Quantitative Determination of mouse TIMP-1 in Serum, Plasma & Cell culture supernatants. Detection Limit: less than 3 pg/ml. 100 assays.BioVision’s Mouse TIMP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP-1. This assay employs an antibody specific for mouse TIMP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of TIMP-1 is typically less than 3 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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5308-100
PANX1 Antibody (NT) Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human, Mouse, Rat Application:Western blot, ELISA Western Blot: 1 - 2 μg/ml, ELISA. However, the optimal conditions should be determined individually.
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BE0020-1-5MG
InVivoMab anti-mouse CD54 (ICAM-1) bioxcell InVivoMab-北京博迈斯科技发展有限公司
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BP0169-50MG
InVivoPlus anti-mouse 4-1BB (CD137) bioxcell InVivoPlus-北京博迈斯科技发展有限公司
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5309-100
NT-4 Antibody Host:Rabbit Antibody Type:Polyclonal Species Reactivity:Human Application:Western blot, Neutralization, ELISA Western blot analysis (0.5-2 μg/ml). Per researchers feedback, it also works well in ELISA (2-3 μg/ml), and neutralization (3-8 μg/ml). However, the optimal conditions should be determined individually.
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