BE0097-50MG
InVivoMab recombinant mouse IgG2a Fc bioxcell InVivoMab-北京博迈斯科技发展有限公司
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BE0097-25MG
InVivoMab recombinant mouse IgG2a Fc bioxcell InVivoMab-北京博迈斯科技发展有限公司
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BE0097-5MG
InVivoMab recombinant mouse IgG2a Fc bioxcell InVivoMab-北京博迈斯科技发展有限公司
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BE0097-1MG
InVivoMab recombinant mouse IgG2a Fc bioxcell InVivoMab-北京博迈斯科技发展有限公司
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BE0097-100MG
InVivoMab recombinant mouse IgG2a Fc bioxcell InVivoMab-北京博迈斯科技发展有限公司
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K4908-100
K4908-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (10X) ? Detection Antibody ? Detector (100X) ? Mouse Nampt Standard (lyophilized,64 ng) ? TMB ? Stop Solution ? Plate Sealers Serum (mouse/ rat)Nampt (Visfatin/PBEF) (mouse/rat)Serum ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of mouse or rat Nampt (Visfatin/PBEF) in serum. Assay range ~0.5 – 32 ng Nampt/ml and detection limit 50 pg/ml. 100 Assays.Visfatin, an adipocytokine that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin, a secretory form of Nampt (nicotinamide phosphoribosy-ltransferase), corresponds to pre-B cell colony-enhancing factor (PBEF), a 52 kDa cytokine expressed in lymphocytes. PBEF is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. The Nampt (Visfatin/PBEF) (mouse/rat) Serum ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat Nampt (Visfatin/PBEF) in serum. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3’,5,5’-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat Nampt. It does not cross-react with human Nampt, human adiponectin, mouse adiponectin, mouse resistin, mouse vaspin, mouse RBP4, mouse GPX3, mouse progranulin, mouse IL-33, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, mouse ANGPTL6, mouse leptin, mouse TNF-α. The assay range is 0.5 – 32 ng Nampt/ml and a detection limit of 50 pg/ml (based on adding two standard deviations to the mean value of the zero standard).
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K4758-100
K4758-100? Serum & plasma ? Cell culture media etc.Nesfatin (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of Nesfatin in Serum, Plasma, Cell culture supernatants etc. Detection range: 0.1-1000 ng/ml. 100 assays.BioVision’s Nesfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Nesfatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Nesfatin antibody, both biotinylated Nesfatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Nesfatin antibody. Uncompeted (bound) biotinylated Nesfatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Nesfatin peptide in the standard or samples. This is due to the competitive binding to Nesfatin antibody between biotinylated Nesfatin peptide and peptides in standard or samples. A standard curve of known concentration of Nesfatin peptide can be established and the concentration of Nesfatin peptide in the samples can be calculated accordingly. EIA-NES-1 detects 420aa Nesfatin protein. No other active isoforms have been reported. The minimum detectable concentration of Nesfatin is 147 pg/ml or 3.09 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4711-100
K4711-100? NRG-1 Ab coated plate ? Human NRG-1 Standard ? Detection antibody (100x) ? HRP Conjugate (100x) ? Sample diluent buffer ? Antibody diluent buffer ? HRP diluent buffer ? TMB substrate ? Stop solution ? Serum and plasma ? Cell culture supernatant Neuregulin-1/NRG1-beta1 (human) ELISA Kit: Rapid & convenient assay for quantitative measurement of native and recombinant human Neuregulin-1 in serum, plasma & cell culture supernatant. Detection range: 62.5 pg/ml – 4000 pg/ml. 100 assays. BioVision’s human NRG-1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human NRG-1 is coated on a 96-well plate. Standards and test samples are added to the wells and NRG-1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal detection antibody from goat specific for NRG-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, HRP conjugate is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is used by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human NRG-1 captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 10 pg/ml. Coefficient of variation: 5% (Intra-assay) and 7% (Inter-assay).
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K4763-100
K4763-100? Notch-1 Microplate (Item A) coated with anti-human Notch-1, 96 wells ? Wash Buffer Concentrate (20x) (Item B) ? Standards (Item C) (recombinant human Notch-1) ? Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) ? Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) ? Detection Antibody Notch-1 (Item F), biotinylated anti-human Notch-1 ? HRP-Streptavidin Concentrate (Item G), 200x concentrated ? TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution ? Stop Solution (Item I), 0.2 M sulfuric acid ? Serum & plasma ? Cell culture supernatants ? Urine Human Notch-1 ELISA Kit: Colorimetric Assay for Quantitative Determination of human Notch-1 in Serum, Plasma, Urine & Cell culture supernatants. Detection Limit: 20 pg/ml. 100 assays. BioVision’s Human Notch-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Notch-1. This assay employs an antibody specific for human Notch-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Notch-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Notch-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Notch-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of Notch-1 is typically less than 20 pg/ml. Detection Range: 20 pg/ml - 7000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
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K4926-100
K4926-100? Pre-coated Microtiter Plate ? Wash Buffer (10X) ? Diluent (5X) ? Lysis Buffer (10X) ? Detection Antibody ? Detector 100X (Hrp conjugated anti-IgG) ? Human NQO1 Standard (lyophilized, 40 ng) ? Human NQO1 QC Sample (lyophilized) ? TMB Substrate Solution ? Stop Solution ? Plate SealersCell lysatesNQO1 (human intracellular) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human NQO1 in Cells. Assay Range 0.313 – 20 ng/ml and Detection limit 100 pg/ml.100 Assays. NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector, both protects the cell from carcinogenic oxidative damage and stabilizes the tumor suppressor p53 protein. At high glycolysis levels NQO1 stabilizes and protects p53 from ubiquitin-independent degradation, a process that is NADH dependent, and also elevates NAD+/NADH levels. NAD+ and NADH play a crucial role in cellular energy metabolism, and a dysregulated NAD+/NADH ratio is implicated in metabolic syndrome. In humans, NQO1 is expressed at high levels in adipocytes and its expression levels are positively correlated with adiposity, glucose tolerance, and liver dysfunction. Thus NQO1 may provide the basis for a new therapy for the treatment of metabolic syndrome. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human NQO1 in cells. A monoclonal antibody specific for NQO1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, NQO1 is recognized by the addition of a purified polyclonal antibody specific for NQO1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of NQO1 in the samples. This ELISA is specific for the measurement of natural and recombinant human NQO1. It does not cross-react with human adiponectin, human RBP4, human Nampt, human vaspin, human progranulin, human resistin, human clusterin, human ANGPTL3, human CTRP5, human IL-33, human leptin, human GPX3, human NMNAT2, human sirtuin 1, human FTO, mouse Nampt, rat Nampt. The assay range is 0.313 – 20 ng NQO1/ml and a detection limit of 100 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
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K4759-100
K4759-100? Serum & plasma ? Cell culture media etc.Obestatin (human/mouse/rat) EIA Kit: Colorimetric Assay for Quantitative Determination of Obestatin in Serum, Plasma, Cell culture supernatants etc. Detection range: 0.1-1000 pg/ml. 100 assays.BioVision’s Obestatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Obestatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Obestatin antibody, both biotinylated Obestatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Obestatin antibody. Uncompeted (bound) biotinylated Obestatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Obestatin peptide in the standard or samples. This is due to the competitive binding to Obestatin antibody between biotinylated Obestatin peptide and peptides in standard or samples. A standard curve of known concentration of Obestatin peptide can be established and the concentration of Obestatin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Obestatin is 0.1 pg/ml. The detection range for the kit is 0.1-1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%.
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K7335-100
K7335-100? Plate coated with OPN MAb ? Assay Diluent A ? Assay Diluent B ? Wash Buffer/Ab Diluent (10x) ? rh OPN Standard (500 ng/vial) ? Detection Ab (100x) ? HRP Conjugate (100x) ? TMB Substrate ? Stop Solution ? Plate Sealer ? Plasma, Serum ? Cell culture supernatants Osteopontin (OPN) (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human Osteopontin (OPN) in serum, plasma and cell culture supernatants. Detection Range: 0.2 to 70 ng/ml. 100 assays. BioVision’s human Osteopontin (OPN) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of human OPN. OPN is a multifunctional, hyperphosphorylated acidic glycoprotein. As a key component of extracellular matrix-associated protein abundantly expressed in bone, OPN is a key regulator of bone mineralization. In addition, OPN is expressed in multiple other cell types including macrophages, smooth muscle cells and hepatocytes. OPN up-regulation occurs upon exposure to pro-inflammatory cytokines (TNF-α, TGF-β etc.) or under pathological conditions such as hypoxia and hyperglycemia. OPN also functions as a chemokine-like protein involved in regulating cell proliferation, inflammatory responses, angiogenesis and metastasis, and is critical in the development of multiple disorders including cardiovascular disease, diabetes, and cancer. The assay employs monoclonal antibody specific for human OPN coated on a 96-well microplate. Standards and samples are pipetted into the wells and OPN present in the sample is bound to the wells by the immobilized antibody. The antibody-captured OPN is then detected by a biotinylated monoclonal antibody. With HRP conjugated Streptavidin and a HRP substrate, amount of OPN in the sample is determined by measuring absorbance of the HRP product at 450 nm with a microplate reader. The sensitivity of the kit is 0.2 ng/ml and detection range is from 0.2 ng to 70 ng/ml. The intra-assay reproducibility as measured by the coefficient of variation (CV) is <5 % & inter-assay CV < 7 %.
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K4829-100
K4829-100? Plate Coated with p53 Ab ? Assay Diluent ? Wash Buffer A (10x) ? Wash Buffer B (10x) ? Wash Buffer C (10x) ? rh p53 Standard ? Detection Antibody (100x) ? Streptavidin-HRP Conjugate (100x) ? TMB Substrate ? Stop Solution ? Plate Sealer ? Cell lysate and cell culture supernatant ? Serum and plasma p53 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human p53 in serum, plasma, cell culture media and cell/tissue lysates. Detection Range: 0.2-25.6 ng/ml. 100 assays. BioVision’s p53 Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of native and recombinant human p53. The p53 protein is encoded by the TP53 gene. It is composed of 393 amino acids but its apparent molecular mass is 53 kDa. p53 binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. It acts as a pivotal suppressor of inappropriate cell proliferation. By initiating suppressive effects through induction of apoptosis, cell senescence, or transient cell-cycle arrest, p53 plays an important role in cancer suppression, developmental regulation, and aging. p53 is found in increased amounts in a wide variety of transformed and tumor cells, where its concentration is increased 5-1000 fold over the concentration in normal cells. Meanwhile, p53 is also frequently mutated or inactivated in about 60% of cancers. The p53 ELISA assay employs an antibody specific for human p53 coated on a 96-well plate. Standards and samples are pipetted into wells and p53 present in the sample is bound to the wells by immobilized antibody. Wells are washed and a biotin-labeled human p53 specific detection antibody is added. After washing away unbound detection antibody, a streptavidin HRP-conjugate is added to the wells. The wells are again washed, a TMB substrate solution is added, and color develops in proportion to the amount of bound p53. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 0.1 ng/ml and detection range is from 0.2 ng/ml to 25.6 ng/ml. Recovery within this range is 86.3%. The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
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K7265-100
K7265-100? PCSK9 Microplate coated with anti-human monoclonal Ab against PCSK9, 96 wells ? Lyophilized recombinant human PCSK9 standard (10 ng/vial) ? Biotinylated anti- human PCSK9 antibody ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB color developing agent (Colorless) ? TMB stop solution ? Serum & plasma (heparin, EDTA) ? Cell culture supernatants PCSK9 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human PCSK9 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s human PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for human PCSK9 coated on a 96-well plate. Standards (NSO, S153-Q692) and test samples are added to the wells and PCSK9 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human PCSK9 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.
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K7266-100
K7266-100? PCSK9 mAb coated plate, 96 wells ? Mouse PCSK9 standard (10 ng/vial) ? Biotinylated anti-mouse PCSK9 Ab ? Avidin-Biotin-Peroxidase Complex (ABC) ? Sample diluent buffer ? Antibody diluent buffer ? ABC diluent buffer ? TMB (Colorless) ? Stop solution ? Serum & plasma (heparin, EDTA) ? Cell culture supernatants PCSK9 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse PCSK9 in serum, plasma & cell culture supernatants. Detection Range: 156 pg/ml - 10,000 pg/ml. 100 assays.BioVision’s mouse PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for mouse PCSK9 coated on a 96-well plate. Standards (NSO, S156Q-694) from murine sarcoma basement membrane and test samples are added to the wells and PCSK9 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse PCSK9 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.
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